薄荷油对雌性布氏田鼠的抗生育效果  

Anti-fertility Effect of Peppermint Oil on Female Lasiopodomys brandtii

作　　者：张兴利[1] 谢晨文 刘筱雪[1] 方帷 李晓[1] 张杰[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川动物》2017年第3期317-324,共8页Sichuan Journal of Zoology

基　　金：公益性行业(农业)科研专项项目(201203041);四川省科技支撑计划项目(2012SZ0045)

摘　　要：我国草地鼠害发生面积呈逐年扩增的趋势。布氏田鼠Lasiopodomys brandtii是内蒙古草原主要害鼠之一,为找到有效控制鼠类数量的方法,本文研究了薄荷油对布氏田鼠的抗生育效果。以性成熟的非越冬布氏田鼠为实验对象,将备用的雌、雄布氏田鼠按2∶1合笼,将妊娠布氏田鼠分为3个不同药物处理组和1个空白对照组,将薄荷油用0.5%羧甲基纤维素钠配置成不同浓度的溶液(750 mg·kg^(-1)、1 000 mg·kg^(-1)、1 500 mg·kg^(-1)),灌胃7 d后剖检,统计其子宫胚胎着床数,对子宫进行组织病理学分析。实验结果表明,薄荷油能有效降低布氏田鼠胚胎着床数(P<0.01)。扫描电子显微镜结果表明,薄荷油使布氏田鼠子宫内膜表面容受性发生了改变;组织病理学结果表明,经薄荷油处理的布氏田鼠子宫形态发生了改变,产生病变;免疫组织化学实验结果显示,药物处理组子宫内的雌激素受体(ERα)、孕激素受体(PR)、基质金属蛋白水解酶(MMP2)及E-钙粘附蛋白(E-cadherin)等相关蛋白的表达水平都出现了异常。这些研究结果表明,薄荷油对布氏田鼠的抗生育作用主要是通过影响调节子宫内ERα、PR、MMP2及E-cadherin等相关蛋白的表达水平来影响其子宫内膜容受性及胚胎着床过程,进而导致胚胎着床失败。因此,薄荷油可作为一种潜在的植物源性不育剂应用于鼠类不育控制。The occurrence area of grassland rodents in China has been increasing year by year. Brandt’s vole { Lasiopodomys brandtii） is a major rodent grassland pest found in Inner Mongolia with high breeding rate. In order to find a more effective way to control the number of rodents, the anti-fertility effect of peppermint oil on Brandfs voles was investiga-ted. The non-overwintering mature female and male Brandt’s voles were mated in a 2：1 ratio. Then the pregnant voles were randomly divided into 3 treatment groups （the concentrations of peppermint oil were ： 750 mg ·kg^-1, 1 000 mg·kg^-1 and 1 500 mg ·kg^-1, respectively） and 1 control group. After intragastric administration for 7 days, the voles were then killed for further autopsy. The number of implanted uterus was counted, and the histopathological study of uterus was carried out.The results showed that peppermint oil could reduce the number of implanted embryos in Brandfs voles. The result of scan-ning electron microscopy showed that the endometrial receptivity of Brandt’s voles administrated by peppermint oil was changed. Histopathological results showed that the uterus morphology of Brandfs voles treated with peppermint oil was also changed. Moreover, the immunohistochemical results showed that the expression levels of estrogen receptor a （ERa） , pro-gesterone receptor （PR） , matrix metalloproteinase-2 （MMP2） , and E-cadherin were all abnormal. These findings showed that the anti-fertility effect of peppermint oil on Brandfs voles was mainly through regulating the expression level of ERa,PR, MMP2 and E-cadherin to affect endometrial receptivity and embryo implantation, and finally led to implantation failure.Therefore, the peppermint oil can be used as a potential plant-derived infertility in rodent sterility control.

关 键 词：布氏田鼠 薄荷油 胚胎着床 抗生育 

分 类 号：S812.6[农业科学—草业科学]