苜蓿雄性不育系MS-4 SSH文库构建及基因表达分析  

Construction of SSH Library and Analysis of Expressed Genes in Male Sterile Line MS-4

作　　者：高翠萍[1] 石凤翎[1] 伊风艳 唐芳[1] 赵彦[1] 蔡丽艳 

机构地区：[1]内蒙古农业大学草地资源教育部重点实验室,呼和浩特010011 [2]内蒙古自治区农牧业科学院,呼和浩特010031 [3]内蒙古自治区林业监测规划院,呼和浩特010020

出　　处：《西北植物学报》2017年第4期665-672,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31302020)

摘　　要：以苜蓿雄性不育系MS-4与可育系花蕾为材料,利用抑制性消减杂交(SSH)技术,分别构建了不育条件下和可育条件下基因差异表达消减cDNA文库,在2个库中分别随机挑选阳性克隆进行测序,经序列比对分析,共获得功能已知的EST序列190条。后经GO功能分类,对推定出的6个相关基因进行荧光定量PCR分析。结果表明:6个基因在苜蓿雄性不育系花蕾不同发育时期中均出现差异表达,推测这6个基因可能与苜蓿雄性育性相关,并且在其育性转换中具有重要作用。The buds of male sterile line of alfalfa MS-4and it＇s maintainer were taken as materials,employed the suppression subtractive hybridization（SSH）technology.Two differential gene expression subtractive cDNA libraries were constructed under sterile and fertile conditions.Positive clones were randomly selected to sequence and analyze by Blast.190 ESTs with known functions were got.6genes were inferred which may be associated with male fertility of alfalfa by GO functional classification.The expression of 6genes were analyzed by fluorescence quantitative PCR,the results showed 6genes had differential expression at different development stages in the male sterile line of alfalfa.It suggested that these 6genes may be related to the male fertility of alfalfa,and play an important role in fertility transformation.

关 键 词：苜蓿 雄性不育性 SSH文库 基因表达量 

分 类 号：Q781[生物学—分子生物学] Q789