外植体类型和培养基中激素浓度对香茶菜愈伤组织诱导的影响  

Effects of explant type and hormone concentration in medium on callus induction of Isodon amethystoides

作　　者：刘芳[1] 陶兴魁[2] 张爱民[3] 滕井通[3] 丁盼盼[3] 薛建平[3] 

机构地区：[1]宿州职业技术学院,安徽宿州234101 [2]宿州学院生物与食品工程学院,安徽宿州234000 [3]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《植物资源与环境学报》2017年第1期113-115,共3页Journal of Plant Resources and Environment

基　　金：安徽省高校省级优秀青年人才基金重点项目(2013SQRL151ZD);淮北市人才培育计划(20140315);宿州学院特色种植业苗种生产工程技术研究中心开放课题(2014XKF19);安徽省高校自然科学研究项目(KJ2014B21)

摘　　要：香茶菜〔Isodon amethystoides （Benth.）H.Hara〕为唇形科（Lamiaceae）香茶菜属〔Isodon （Schrader ex Benth.）Spach〕植湖北和广西等省（自治区）,为安徽宿州著名的传统药食两用植物，以茎叶入药。Effects of explant type( leaf and stem segment) and hormone concentration( 0. 1,0. 2 and 0. 3 mg·L^(-1) 6-BA;1. 0,1. 5 and 2. 0 mg·L^(-1)2,4-D) in medium on callus induction of Isodon amethystoides( Benth.) H. Hara were studied by using single-factor and L_9( 3~2) orthogonal experiments. The results show that compared with callus induction from leaves,calli can be induced from stem segments within a short time,which are loose and large in quantity. The results of orthogonal experiment show that the callus induction rate of stem segments of I. amethystoides varies from 72% to 93% in different media,in which,mass concentration of 2,4-D has a significant( P<0. 05) effect on callus induction rate,while that of 6-BA has no significant effect. According to the results,MS medium( containing 30 g·L^(-1) sucrose and 7 g·L^(-1) agar,pH 5. 8) with 0. 1 mg·L^(-1) 6-BA and 1. 5 mg·L^(-1) 2,4-D is considered as an optimum medium for callus induction from stem segments of I. amethystoides.

关 键 词：香茶菜 外植体类型 激素浓度 正交实验 

分 类 号：Q813.12[生物学—生物工程] Q945