骨关节炎患者外周血单个核细胞miR-335-5p表达及其意义  

Expression and significance of miR-335-5p in the peripheral blood mononuclear cells of patients with osteoarthritis

作　　者：何伟珍[1] 尹志华[1] 徐晓东[1] 叶志中[1] 郭佩[1] 

机构地区：[1]中山大学附属第八医院香蜜湖分院,深圳518040

出　　处：《新医学》2017年第5期323-325,共3页Journal of New Medicine

基　　金：国家自然科学基金(81102266;81301529);深圳市科技计划项目(JCYJ20140416094256891);深圳市医学重点学科建设资助项目(2005C10)

摘　　要：目的探讨微小RNA-335-5p(miR-335-5p)在骨关节炎患者外周血单个核细胞(PBMC)中的表达及其意义。方法收集50例骨关节炎患者及30例健康志愿者的外周抗凝血,分离PBMC,实时定量PCR检测miR-335-5p表达水平。分析miR-335-5p与骨关节炎患者的年龄、BMI及Kellgren-Lawrence X线分级之间的相关性。结果骨关节炎患者PBMC中miR-335-5p表达水平低于健康对照组(0.00090±0.00008 vs.0.00278±0.00025,P<0.001),骨关节炎患者PBMC中miR-335-5p表达水平与其年龄(r=-0.763,P<0.001)、BMI(r=-0.440,P<0.001)呈负相关,与KellgrenLawrence X线分级无关(P>0.05)。结论 miR-335-5p在骨关节炎患者PBMC中表达下调,与年龄、BMI呈负相关,提示其可能是骨关节炎的保护性基因。Objective To investigate the expression and significance of microRNA-335-5p （ miR- 335-5p） in the peripheral blood mononuclear cells （PBMCs） of patients with osteoarthritis. Methods The peripheral blood samples were collected from 50 osteoarthritis patients and 30 healthy controls. The PBMCs were isolated from the blood samples. The expression level of miR-335-5p was detected using quantitative real-time reverse transcription PCR （qRT-PCR）. The correlation between miR-335-5p and age, body mass index （BMI） and Kellgren-Lawrence classification for knee osteoarthritis was analyzed. Results The expression of miR-335-5p in the PBMCs of osteoarthritis patients was significantly down-regulated compared with that in the healthy controls （0. 00090 ±0. 00008 vs.0. 00278 ±0. 00025,P 〈0. 001）. In the PBMCs of osteoarthritis pa-tients, the expression of miR-335-5p was negatively correlated with the age （r =-0. 763 , P〈0. 001 ） and BMI （r = -0. 440, P 〈0. 001 ） , whereas it was not correlated with the Kellgren-Lawrence classification for knee osteoarthritis （P 〉0. 05）. Conclusions The expression of miR-335-5p is significantly down-regulated in the PBMCs of osteoarthritis patients. Moreover, it is negatively correlated with age and BMI, suggesting that miR-335-5p might be a protective gene for osteoarthritis.

关 键 词：骨关节炎 微小RNA-335-5P 外周血单个核细胞 

分 类 号：R684.3[医药卫生—骨科学]