含铜节育器植入家兔体内血清铜离子含量测定  

Determination of Copper Ion in Rabbit with Implantation of Copper Intrauterine Device

作　　者：徐小玲 何开勇 潘望平 吕晓君 胡远华 

机构地区：[1]湖北省食品药品监督检验研究院,武汉430064

出　　处：《医药导报》2017年第6期614-616,共3页Herald of Medicine

摘　　要：目的建立植入含铜节育器家兔血清铜离子含量测定方法。方法采用硝酸对血清样品进行微波消解制样,钪(Sc)、铟(In)作为内标补偿基体效应,采用电感耦合等离子体质谱法(ICP-MS法)测定兔血清中铜的含量,分析统计铜离子的释放和代谢规律。结果家兔放置含铜节育器后,在不同采血时间点,血清铜离子浓度维持在较低且恒定的范围。加样回收率为98.7%~113.3%,RSD均<5%。结论该方法操作简便、快速,结果可靠,可用于家兔血清铜离子的测定。Objective To develop a method for determination of serumal copper ion in rabbit with implantation of copper intrauterine device. Methods At different time points after implantation,the serumal copper ion concentrations were determined by microwave digestion-inductively coupled plasma mass spectrometry（ ICP-MS） using scandium（ Sc） and indium（ In） as internal standards to compensate sample matrix effects,and the pharmacokinetics parameters were calculated in order to reflect copper ion release and metabolic rule. Results The serumal copper ion concentrations were kept at a low and stable level. The recoveries were in the range of 98. 7%-113. 3%,with the relative standard deviations of less than 5. 0%.Conclusion The analytical method is simple,fast,and can be used for the determination of serumal copper ion in rabbit.

关 键 词：含铜节育器 铜离子 电感耦合等离子体质谱法 

分 类 号：R979.2[医药卫生—药品] R965[医药卫生—药学]