金乌健骨方对类风湿关节炎滑膜细胞IL-17和ACT1及TRAF6表达的影响  

Effect of Jinwu Jiangu Decoction on IL-17,ACT1,TRAF6 in Synovial Cells of Rheumatoid Arthritis

作　　者：马武开[1] 宁乔怡 李东晓[1] 姚血明[1] 侯雷[1] 梁江[1] 黄颖[1] 周静[1] 

机构地区：[1]贵阳中医学院第二附属医院风湿免疫科,贵阳550003

出　　处：《医药导报》2017年第6期617-621,共5页Herald of Medicine

基　　金：国家自然科学基金资助项目(81360580);贵州省社会发展攻关计划课题(黔科合SY[2010]3102号);贵州省省长基金课题[黔省专合字(2012)33];贵州省科技创新人才团队项目[黔科合人才团队(2013)4033]

摘　　要：目的探讨苗药金乌健骨方含药血清冻干粉对人类风湿关节炎成纤维样滑膜细胞(RA-HFLS)白细胞介素17(IL-17)、核因子κB激活蛋白1抗体(ACT1)、肿瘤坏死因子受体相关因子6(TRAF6)表达的影响。方法大白兔随机分为空白对照组,金乌健骨方大、中、小剂量组,雷公藤多苷组和泼尼松组,每组各6只。金乌健骨方大、中、小剂量组分别按生药量14.4,4.8,2.4 g·kg^(-1)灌胃给药,雷公藤多苷组和泼尼松组按成人用量的等效剂量给药,空白对照组灌服等容量0.9%氯化钠溶液,连续7 d,取血制备含药血清冻干粉。建立原代RA-HFLS细胞模型,含药血清冻干粉进行干预治疗,酶联免疫吸附测定(ELISA)法检测含药血清冻干粉对IL-17分泌的影响,反转录-聚合酶链反应(RT-PCR)检测含药血清冻干粉对ACT1、TRAF6 mRNA表达的影响。结果与空白对照组比较,各给药组细胞培养上清液中IL-17分泌下降(均P<0.01),以金乌健骨方大、中剂量组最为明显,大剂量组对IL-17的分泌下调作用优于雷公藤多苷组(P<0.01);与空白对照组比较,各给药组TRAF6、ACT1 mRNA的表达均不同程度下调(均P<0.01),以金乌健骨方大剂量组最为明显,但与雷公藤多苷组和泼尼松组比较,差异无统计学意义(P>0.05)。结论金乌健骨方含药血清冻干粉能降低RA-HFLS IL-17的分泌,下调RA-HFLS ACT1、TRAF6 mRNA的表达。Objective To investigate the effect of Miao medicine Jinwu Jiangu decoction containing serum freeze-dried powder on levels of IL-17,ACT1 and TRAF6 in human rheumatoid arthritis fibroblast like synoviocytes（ RA-HFLS）. Methods Rabbits were randomly divided into blank control group（ recieving normal saline of the same volume）,Jinwu Jiangu decoction high-dose,medium-dose and low-dose group（ intragastrically administrated with Jinwu Jiangu decoction at doses of 14. 4,4. 8 and2. 4 g·kg^（-1）,respectively）,tripterygium glycosides group and prednisone group（ treated with human equivalent dosage）. RAHFLS primary cell model was established in the experiment. ELISA method was used to detect effect of lyophilized powder on IL-17 secretion. Expression of ACT1,TRAF6 mRNA was detected by RT-PCR. Results Compared with the blank control gorup,IL-17 in the supernatant of each medication administration group was significantly decreased（ all P〈 0. 01）,and it was decreased most significantly in Jinwu Jiangu decoction high-dose and medium-dose group. IL-17 was down-regulated more significantly in high-dose group than that in tripterygium glycosides group（ P〈 0. 01）. Compared with the blank control group,TRAF6 and ACT1 mRNA expression level of each medication administration group were significantly decreased（ all P 〈0. 01）,and in the high-dose group that were decreased most significantly,but not significantly different as compared with tripterygium glycosides group and prednisone group（ P 〉0. 05）. Conclusion Freeze-dried powder of Jinwu Jiangu decoction can decrease the secretion of IL-17 and down-regulate expression of ACT1,TRAF6 with RA-HFLS.

关 键 词：金乌健骨方 关节炎 类风湿 滑膜细胞 白细胞介素-17 

分 类 号：R289.71[医药卫生—方剂学]