羧甲基壳聚糖水凝胶的合成及其对人皮肤成纤维细胞增殖的影响  

Synthesis and characterization of carboxymethyl chitosan hydrogels and their effect on proliferation of fibroblasts

作　　者：王栋[1] 何静[1] 吴方[1] 

机构地区：[1]四川大学国家生物医学材料工程技术研究中心,成都610000

出　　处：《中国科技论文》2017年第6期623-626,共4页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20120181110058);四川省科技厅苗子工程资助项目(2016RZ0032)

摘　　要：制备了一系列羧甲基壳聚糖(carboxymethyl chitosan,CMCS)-京尼平水凝胶。通过向CMCS中加入不同浓度的氯化钙溶液,然后与京尼平进行交联反应合成水凝胶,用三硝基苯磺酸(TNBS)法测定自由氨基含量,考察添加钙离子的浓度对CMCS水凝胶中自由氨基含量的影响。通过在水凝胶上培养人皮肤成纤维细胞,用CCK-8法测定细胞增殖结果,考察了水凝胶中自由氨基浓度对人皮肤成纤维细胞增殖的影响。结果表明,随着添加的钙离子浓度的提高,水凝胶中自由氨基的浓度降低,人成纤维细胞增殖能力提高。A series of carboxymethyl chitosan（CMCS）-genipin hydrogel have been synthesized by adding different concentration of calcium chloride solution.TNBS method was applied to evaluate the effect of the different concentrations of calcium ions on free amino content in the CMCS hydrogel.CCK-8method was applied to evaluate the effect of the concentrations of free amino in hydrogel on human skin fibroblast proliferation.The results demonstrate that the content of the free amino groups decreases with the increasing concentration of calcium added in the hydrogel,which promotes the proliferation of fibroblasts.Therefore,CMCSgenipin hydrogel may be a potential wound dressings.

关 键 词：生物材料 水凝胶 羧甲基壳聚糖 钙离子 自由氨基 成纤维细胞 增殖 

分 类 号：R318.08[医药卫生—生物医学工程]