细胞污染及检测鉴定  

Detection and Identification of Cell Contamination

作　　者：陈琳[1,2] 陈鲤群[1,2] 

机构地区：[1]福州大学应用基因组学研究所,福州350108 [2]福州大学生物科学与工程学院,福州350108

出　　处：《中国细胞生物学学报》2017年第4期496-503,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31500616)资助的课题~~

摘　　要：细胞作为重要的实验模型广泛应用于各类实验,几乎所有科研实验室中都需进行细胞培养。细胞培养过程中最大的威胁就是细胞污染,凡是混入细胞培养环境中,对细胞生存有害的成分和造成细胞不纯的异物都应视为污染。细胞一旦遭到污染,所有的实验结果都会变得毫无意义。因此,及时检测并鉴定出所培养的细胞是否被污染至关重要。该文总结了细菌污染、真菌污染、支原体污染、细胞交叉污染和鞘氨醇单胞菌污染这五类细胞污染及其检测方法,并提出了相关污染的预防措施及几种常见清除方法以供参考。Cell lines are used extensively all research laboratories cultured cells. The most and impurities which mixed into the cell culture in various research as an important experimental model. Almost serious threat in cell culture is cell contamination. Ingredients environment, harmful to cells or caused cell impurities should be considered as contamination. Once the cells have been contaminated, all the results will be meaningless. Therefore, detection and identification of cell contamination in time is essential. This paper introduces the bacterial contamination, fungal contamination, mycoplasma contamination, cell cross-contamination, Sphingomonas pollution and some detection methods thereof. Additionally, several preventive measures and common cleaning methods are put forward so as to provide a reference for future reference.

关 键 词：细胞污染 污染分类 鉴定 

分 类 号：Q-33[生物学]