利用基因敲除小鼠研究雄激素受体对骨骼肌影响的研究进展  

Advance in the Influences of Androgen Receptor on Skeletal Muscle Based on AR Gene Knockout Mice

作　　者：鲁林[1] 王晓慧[1] 

机构地区：[1]上海体育学院运动科学学院,上海200438

出　　处：《中国细胞生物学学报》2017年第4期529-536,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31271274)资助的课题~~

摘　　要：雄激素除了促进男性性器官成熟和维持男性第一、第二性征之外,在提高肌肉质量和力量方面也具有非常重要的作用。雄激素主要通过与雄激素受体(androgen receptor,AR)结合来发挥其生物学功能。近年来,雄激素/AR在骨骼肌肥大、肌纤维类型以及老年性肌肉丢失中的重要作用已得到广泛关注。尽管有睾丸切除动物模型,但雄激素及AR的作用机制远未被完全阐明。利用Cre/lox P系统建立的组织特异性、细胞特异性和诱导性的AR条件性敲除小鼠为研究雄激素/AR在骨骼肌中的作用及机制提供了更好的动物模型。该文就AR敲除小鼠(AR knockout,ARKO)骨骼肌质量、肌纤维类型、结构和力量的改变和机制以及正确解释ARKO小鼠表型需要注意的问题等作一综述。Apart from producing and maintaining primary and secondary sexual characteristics, androgen plays a pivotal role in regulating the mass and strength of skeletal muscles. Androgen mainly exerts its biological role through binding the androgen receptor（AR）. Though the studies in the model animals of orchiectomy and in vitro culture of skeletal muscles and cells, androgen/AR signal pathway is still far from fully elucidated. In recent years, global AR knockout（ARKO） mice and tissue-specific, cell-specific or inducible ARKO mice established by Cre/lox P system provided a better platform to elucidate the effects and mechanisms of androgen/AR signal pathway in regulating the mass and strength of skeletal muscles. This review focuses on the changes and underlying mechanisms of mass, fiber type, structure and strength of skeletal muscles that have been gained from ARKO mice, as well as the attentions that should be paid to accurately interpretate the phenotypes of Crelox P mouse models.

关 键 词：雄激素受体 CRE/LOX P系统 基因敲除小鼠 骨骼肌 

分 类 号：R339.2[医药卫生—人体生理学]