活血凉血法对氧糖剥夺大鼠星形胶质细胞内神经营养因子的影响  

Effect of Blood-Activating and Cooling-blood Method on Neurotrophin in Astrocytes of Oxygen-glucose Deprived Rats

作　　者：石冬燕[1] 朱元[1] 常诚[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中国中医急症》2017年第5期756-760,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81673759);江苏省六大高峰人才资助项目(2013WS-034)

摘　　要：目的观察活血凉血法作用于脑缺血缺氧大鼠脑微血管内皮细胞(BMEC)后的条件培养基对星形胶质细胞(AS)内神经营养因子(NT)的影响。方法采用氧糖剥夺法获得正常、激活、激活合通络救脑注射液处理、损伤、损伤合通络救脑注射液处理5个不同状态的BMEC条件培养基。同法建立AS损伤模型并分成7组,加入相应BMEC条件培养基:正常对照组(N)、模型组(I)、条件培养基处理的正常组(NCM)、条件培养基处理的激活组(ACM)、条件培养基处理的激活给药组(ACM-T)、条件培养基处理的损伤组(ICM)、条件培养基处理的损伤给药组(ICM-T)。观察AS活性,并检测BDNF、GDNF的表达量。结果正常、激活和损伤状态的BMEC条件培养基均可提高受损AS活性,并显著升高其分泌BDNF、GDNF的量,其促进程度分别为激活组>正常组>损伤组。凉血活血之通络救脑注射液作用于激活BMEC后,其条件培养基具有促进AS分泌BDNF、GDNF的功能。结论活血凉血的通络救脑注射液可通过提高受损星型胶质细胞活性并促进其分泌NT来减轻脑缺血缺氧时神经细胞的损伤,促进神经细胞功能的恢复,其可能是治疗脑缺血缺氧的潜在药物。Objective： To study the effect of blood-activating and cooling-blood method on neurotrophin in astrocytes of oxygen-glucose deprived rats by conditioned medium of rat brain microvascular endothelial cells （BMEC）. Methods： Five different states of BMEC conditions medium with oxygen-glucose-deprivation legal system called normal,active,activate merge Tongluo Jiunao Injection process,damage,and injury combined Tongluo Jiunao Injection process were made. The injured AS models were made in the same way and divided it into seven groups,being added the corresponding BMEC conditioned medium,namely normal controlled group （N）, model group （I）, the normal group treated with conditioned medium （NCM）, activated group treated with con- ditioned medium （ACM）,activated conditioned medium administration group treated group （ACM-T）,injured group treated with conditioned medium （ICM）,damage administration group treated with conditioned medium （ICM-T）. The activity of AS was observed and the amount of BDNF ＆ GDNF came from AS was detected. Re- suits： All of the normal,active and damage BMEC conditions states could significantly increase the activity of impaired AS and then significantly increase its secretion of BDNF ＆ GDNF, the sequence of promotive effect was the activated group〉 the normal group〉 the injured group. When Tongluo Jiunao Injection was acted on the acti- vated BMEC as a conditioned medium,it could promote the secretion of neurotrophin from AS. Conclusion： Tongluo Jiunao Injection has the blood-activating and cooling-blood function. It can significantly improve the ac- tivity of AS and promote the secretion of neurotrophin to reduce damage to the nerve cells when cerebral hypoxiaischemia happens,promote the,recovery of nerve cell function which shows that it is a potential drug to treat cere- bral hypoxia-ischemia.

关 键 词：活血凉血法 脑微血管内皮细胞 星形胶质细胞 神经生长因子 通络救脑注射液 

分 类 号：R285.5[医药卫生—中药学]