肾衰饮对慢性肾功能衰竭大鼠ATF6/CHOP通路的作用研究  

Effects of Shenshuaiyin Decoction on ATF6/CHOP Pathway in Kidney Tissues of Chronic Renal Failure Rats

作　　者：刘烨[1] 马晓燕[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《中国中医急症》2017年第5期768-770,796,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81373527);沈阳科学技术计划项目(F13-220-9-18)

摘　　要：目的观察肾衰饮对慢性肾功能衰竭大鼠肾脏ATF6/CHOP通路的影响,探析肾衰饮延缓慢性肾功能衰竭的作用机制。方法 52只SD大鼠,按体质量分层随机抽取10只为正常组,其他大鼠采用腺嘌呤灌胃法模型3周。造模成功后随机分为模型组、肾衰饮组和尿毒清组。肾衰饮组和尿毒清组分别给予肾衰饮煎剂和尿毒清颗粒连续灌胃4周。全自动生化分析仪检测血肌酐、尿素氮含量,HE染色观察各组大鼠肾组织病理变化,Western Blot技术检测大鼠肾脏ATF6、CHOP、Bcl-2、Bax、Caspase-3蛋白表达情况。结果与正常组比较,模型组Scr、BUN水平显著升高(P<0.01);与模型组比较,肾衰饮组和尿毒清组大鼠Scr、BUN水平均显著降低(P<0.01)。HE染色提示:肾衰饮及尿毒清能够改善模型组大鼠肾脏病理学改变。Western Blot结果提示:与正常组比较,模型组大鼠肾脏ATF6、CHOP、Caspase-3蛋白表达水平显著升高(P<0.01),Bcl-2/Bax水平显著降低(P<0.01);与模型组比较,肾衰饮组大鼠ATF6、CHOP、Caspase-3蛋白表达水平显著降低(P<0.05或P<0.01),Bcl-2/Bax水平显著升高(P<0.01)。结论肾衰饮可能通过影响ATF6/CHOP通路,减少肾组织细胞凋亡,保护受损肾脏功能。Objective： To discuss the pharmacology mechanism of Shenshuaiyin Decoction delaying the kidney failure by observing the effect of Shenshuaiyin Decoction on ATF6/CHOP pathway in kidney tissues of chronic renal failure rats. Methods： From 52 SD rats ,8 rats were randomly selected as the normal group. The other rats were taken as renal failure models by adenine gavage method for 3 weeks. After successful modeling,the modeled rats were randomly divided into model group,Shenshuaiyin Decoction group and Niaoduqing group. The Shenshuaiyin Decoction group and Niaoduqing group rats were given Shenshuaiyin Decoction and Niaoduqing gavage for 4 weeks. SCr and BUN were tested using the automatic biochemical analyzer. The renal pathological changes in rats of each group were observed by HE staining method. ATF6,CHOP,Bcl-2,Bax and Caspase-3 protein expression were tested with Western blot methods. Results： Compared with the normal group, the Scr and BUN level in the model group increased significantly （P〈 0.01 ）. Compared with the model group,the Scr and BUN level in Shenshuaiyin Decoction group and Niaoduqing group decreased significantly （P 〈 0.01 ）. HE staining results showed that Shenshuaiyin Decoction and Niaoduqing could improve the renal pathological changes in rats of the model group. Western blot results showed that compared with the normal group,the expression level of ATF6, CHOP, Caspase3 increased significantly （P〈 0.01 ） and the expression levels of Bcl-2/Bax decreased significantly （P〈 0.01 ） in the model group. Compared with the model group ,the expression level of ATF6 ,CHOP,Caspase-3 decreased significantly （P〈0.05 or P〈0.01） and the expression levels of Bcl-2/Bax increased significantly （P〈 0.01 ） in Shenshuaiyin Decoction group. Conclusion： Shenshuaiyin Decoction plays an important biological role in reducing renal tissue cell apoptosis and improving the impaired renal function via regulating ATF6/CHOP pathway of chronic renal failure rats.

关 键 词：慢性肾功能衰竭 肾衰饮 内质网应激 ATF6 CHOP 凋 亡 

分 类 号：R285.5[医药卫生—中药学]