熊果酸对金黄色葡萄球菌生物膜形成的影响  

The inhibition effects of ursolic acid on biofilm formation of Staphylococcus aureus

作　　者：李洋[1,2,3] 徐硕超 刘广锋[4] 冯娟[4] 管淑玉[1,2,3] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]国家中医药管理局中药数字化质量评价技术重点实验室,广东广州510006 [3]广东高校中药质量工程技术研究中心,广东广州510006 [4]中国水产科学研究院南海水产研究所,广东广州510300

出　　处：《广东药科大学学报》2017年第2期158-161,共4页Journal of Guangdong Pharmaceutical University

基　　金：国家自然科学基金项目(81001702);广州市科技计划产学研协同创新重大专项(201508020016);海南省科技兴海专项(2015XH03)

摘　　要：目的研究熊果酸对金黄色葡萄球菌(S.aureus)生物膜形成的影响。方法二倍稀释法测定熊果酸与左氧氟沙星对S.aureus的最低抑菌浓度(MIC),采用紫外法测定金黄色葡萄球菌的生长曲线,结晶紫染色法测定生物膜生成量,倒置显微镜下观察生物膜结构。结果熊果酸对S.aureus的MIC为0.27mmol/L,左氧氟沙星的为0.000 6 mmol/L,不同浓度熊果酸不仅对S.aureus浮游菌的生长有抑制作用,且能显著抑制其生物膜的形成,并呈浓度依赖性;与左氧氟沙星和熊果酸单独应用相比,熊果酸和左氧氟沙星联合用药更能显著抑制S.aureus生物膜形成,且差异具有统计学意义(P<0.01)。结论熊果酸与左氧氟沙星联用对抑制S.aureus生物膜形成有增效作用。Objective To study the effect of ursolic acid on the biofilm formation of Staphylococcus aureus. Methods Double dilution method was used to determine the minimum inhibitory concentration（MIC） of ursolic acid and levofloxacin. The growth curves of S. aureus of the normal group and ursolic acid group were detected by using a spectrophotometer, S. aureus biofilm was examined by crystal violet assay, and the biofihn structure was observed under optical microscope. Results The minimum inhibitory concentration （MIC） of ursolic acid and levofloxacin for S. aureus was 0.27 mmol/L and 0.000 6 mmol/L, Ursolic acid had good inhibitory activity for the early stages of biofilm formation in a dose-dependent manner, when combined with levofloxacin, ursolic acid resulted in a significant decrease in the established biofilm biomass compared to levofloxacin or ursolic acid used alone（ P〈 0.01）. Conclusion Ursolic acid and levofloxacin have synergistic inhibitory effect on S. aureus biofilm formation.

关 键 词：熊果酸 金黄色葡萄球菌 生物膜 左氧氟沙星 

分 类 号：R285[医药卫生—中药学]