半仿生及超声波辅助半仿生法提取杜仲叶黄酮  

Semi Bionic Extraction and Ultrasonic-Assisted Semi Bionic Extraction of Flavonoids from Eucommia Ulmoides

作　　者：邓佑林[1] 唐石[1,2] 王美春[1] 杨盼盼[1] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000 [2]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000

出　　处：《吉首大学学报（自然科学版）》2017年第2期66-70,共5页Journal of Jishou University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(21462017);林产化工工程湖南省重点实验室开放基金项目(JDZ201402);武陵山区特色植物资源开发与应用湖南省研究生培养创新基地开放项目(2014KFXM06)

摘　　要：探讨半仿生法及超声波辅助半仿生法提取杜仲黄酮的实验条件.半仿生法考察提取温度、提取时间、料液比对黄酮提取率的影响.以半仿生法提取在超声波协同作用的基础上,采用正交试验、单因素考察超声时间、超声温度、料液比对黄酮提取率的影响.结果表明,半仿生法在提取时间1.5h,提取温度60℃,料液比1∶20(g∶mL)时,黄酮提取率高达325.0mg/g;超声波辅助法提取的最佳条件为时间50min,温度65℃,料液比1∶40(g∶mL),黄酮提取率为264.4mg/g.The experimental conditions of semi bionic extraction and ultrasonic-assisted semi bionic extraction of flavonoids from eucommia ulmoides were explored.For semi bionic extraction,the effect of extracting temperature,extracting time,and solid-liquid ratio on extraction efficiency was examined.For ultrasonic-assisted semi bionic extraction,the effect of ultrasonic time,ultrasonic temperature,and solid-liquid ratio on extraction efficiency was examined through the orthogonal test and single factor study.The experimental results showed that semi bionic extraction of 1∶20（g∶mL）solid-liquid for 1.5hat 60 ℃ yielded 325.0mg/g flavonoid.The optimum conditions of ultrasonic-assisted extraction were extraction of 1∶40（g∶mL）solid-liquid for 50 min at 65 ℃,and the content of flavonoids reached 264.4mg/g.

关 键 词：杜仲 黄酮 半仿生提取法 超声波辅助 

分 类 号：TQ46[化学工程—制药化工] R284[医药卫生—中药学]