A pH-Activatable Fluorescent Aptamer Probe for Imaging of Target Cancer Cells  

作　　者：ZHOU Yan FU Boshi XIAO Heng 

机构地区：[1]Department of Gastroenterology, Zhongnan Hospital of Wuhan University [2]College of Chemistry and Molecular Sciences, Wuhan University [3]Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology

出　　处：《Wuhan University Journal of Natural Sciences》2017年第3期247-251,共5页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Program on Key Basic Research Project(973 Program)(2012CB720600,2012CB720603,2012CB720605);the National Natural Science Foundation of China(21432008,91413109,81373256,91213302)

摘　　要：Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment. Herein, we developed a novel method to detect and evaluate biomarkers on cancer cell surface. Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells, we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction. The spectral characteristics of fluorophore naphthalimide has a good pH dependence. By this way, nucleolin-overexpressed cell could be discriminated from normal cell. Further, this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin-embedded（FFPE） tissue specimens. It is considered that our method has the potential to be applied in medical detection.Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment. Herein, we developed a novel method to detect and evaluate biomarkers on cancer cell surface. Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells, we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction. The spectral characteristics of fluorophore naphthalimide has a good pH dependence. By this way, nucleolin-overexpressed cell could be discriminated from normal cell. Further, this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin-embedded（FFPE） tissue specimens. It is considered that our method has the potential to be applied in medical detection.

关 键 词：aptamer fluorescence pH-activatable probe cancer cell imaging 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]