Association between Serum 25-hydroxy Vitamin D Concentration and Taql Vitamin D Receptor Gene Polymorphism among Jordanian Females with Breast Cancer  

作　　者：Manar Fayiz Atoum Yasmeen Mohammad Al-Khatib 

机构地区：[1]Department of Medical Laboratory Sciences, Faculty of Allied Health, Hashemite University, Zarqa 13115, Jordan

出　　处：《Chinese Medical Journal》2017年第9期1074-1078,共5页中华医学杂志（英文版）

摘　　要：Background： Breast cancer is the most common type of cancer among females. Genetic polymorphisms might have a role in carcinogencsis. The aim of this study was to determine whether C to T base substitution within Taql Vitamin D receptor （VDR） gene （rs731236） in exon 9 was a risk factor among patients with breast cancer. Methods： Peripheral blood was drawn from 122 Jordanian breast cancer patients and 100 healthy Jordanian volunteers in Al-Bashecr Hospital during the summer months （from June to November of 2013, 2014, and 2015）. DNA was amplified using polymerase chain reaction （PCR）, followed by Taql restriction enzyme digestion. Quantification of serum 25-hydroxy Vitamin D （25[OH]D） level was determined by competitive immunoassay Elecsys. Results： Genotypic frequencies for Taql TT, Tt, and tt genotypes were 41%, 46%, and 13% for breast cancer compared to 42%, 50%. and 8% for control, respectively. Vitamin D serum level was significantly lower in the breast cancer patients （8.1 ± 0.3 ng/ml） compared to the control group （21.2± 0.6 ng/ml; P =0.001）. This study showed an inverse association between 25（OH）D serum level and brcasl cancer risk （odds ratio [OR], 22.72, 95% confidence interval [C/], 10.06 51.29）. Conclusions： An inverse association was found between 25（OH）D serum level and breast cancer risk. Statistical difference was also found between different VDR Taql genotypes and circulating levels of 25（OH）D among Jordanian females with breast cancer.Background： Breast cancer is the most common type of cancer among females. Genetic polymorphisms might have a role in carcinogencsis. The aim of this study was to determine whether C to T base substitution within Taql Vitamin D receptor （VDR） gene （rs731236） in exon 9 was a risk factor among patients with breast cancer. Methods： Peripheral blood was drawn from 122 Jordanian breast cancer patients and 100 healthy Jordanian volunteers in Al-Bashecr Hospital during the summer months （from June to November of 2013, 2014, and 2015）. DNA was amplified using polymerase chain reaction （PCR）, followed by Taql restriction enzyme digestion. Quantification of serum 25-hydroxy Vitamin D （25[OH]D） level was determined by competitive immunoassay Elecsys. Results： Genotypic frequencies for Taql TT, Tt, and tt genotypes were 41%, 46%, and 13% for breast cancer compared to 42%, 50%. and 8% for control, respectively. Vitamin D serum level was significantly lower in the breast cancer patients （8.1 ± 0.3 ng/ml） compared to the control group （21.2± 0.6 ng/ml; P =0.001）. This study showed an inverse association between 25（OH）D serum level and brcasl cancer risk （odds ratio [OR], 22.72, 95% confidence interval [C/], 10.06 51.29）. Conclusions： An inverse association was found between 25（OH）D serum level and breast cancer risk. Statistical difference was also found between different VDR Taql genotypes and circulating levels of 25（OH）D among Jordanian females with breast cancer.

关 键 词：Breast Cancer GENOTYPES Taql Vitamin D Receptor 

分 类 号：Q565[生物学—生物化学] Q346.5