慢性束缚应激对小鼠学习记忆能力的影响  

Effect of chronic restraint stress on learning and memory of mice

作　　者：邵亚杰[1,2] 唐娅辉 曾贵荣 张妙红 周仕达 周雅娜[1] 贾韵涛 姜德建[1,2,3] 

机构地区：[1]湖南师范大学生命科学学院生态安全检测与评价重点实验室,长沙410006 [2]湖南省药物安全评价研究中心,新药药效与安全性评价湖南省重点实验室,长沙410331 [3]湖南省中药粉体与创新药物省部共建国家重点实验室培育基地,长沙410007

出　　处：《中南药学》2017年第4期455-458,共4页Central South Pharmacy

基　　金：湖湘青年创新人才基金(编号:2014);中国航天员中心人因工程重点实验室开放课题(No.2012ZX09J12201-006)

摘　　要：目的研究慢性束缚应激对小鼠学习记忆能力的影响。方法雄性ICR小鼠30只,按体重随机分为3组:正常对照组、束缚模型组和解束缚组。束缚模型组动物采用束缚结合孤养建立慢性应激模型,每日束缚10 h,连续56 d;解束缚组同模型组造模28 d后不再束缚和孤养,继续喂养28 d。实验期间对各组动物进行Morris水迷宫检测空间学习记忆。行为学检测后取海马组织进行组织病理检查,ELISA法检测匀浆组织碱性成纤维生长因子2(FGF2)水平。结果造模26、27、54、55 d时,束缚模型组和解束缚组小鼠定位航行潜伏期均明显增加,造模28 d、56 d后空间探索经过平台次数明显减少(P<0.05或P<0.01),造模56 d后束缚模型组经过平台次数与解束缚组比较明显减少(P<0.01)。造模后束缚模型组和解束缚组小鼠海马组织FGF2均明显降低(P<0.05),且海马组织出现不同程度的细胞排列紊乱,椎体细胞坏死等病理改变。结论慢性束缚应激能明显损伤小鼠空间记忆功能,其损伤机制可能与降低海马FGF2含量,损伤海马结构有关。Objective To investigate the effect of chronic restraint stress on learning and memory of mice. Methods Totally 30 male ICR mice were randomly divided into 3 groups according to their weight： a normal control group, a restraint model group, and a restraint removal group. The restraint model group was establelished by restraint stress and separated feeding, 10 hours daily for 56 days. The mice in the restraint removal group were restraint stress and separated feeding for 28 days, and continued breeding for 28 days. All mice were tested the spatial memory by morris water maze. Pathological examination and the level of alkaline growth factor 2 （FGF2） were detected in the hippocampus tissue after behavioral test. Results The spatial memory test showed that the restraint model group and the remove restraint group exhibited longer escape latency than the normal control group （P 〈 0.05 or P 〈 0.01） on 26th, 27th, 54th, 55th day. The restraint removal group showed more crossing platform number （P 〈 0.01） on 28th and 56th day. The content of hippocampus FGF2 decreased significantly in the restraint model group and the restraint removal group （P 〈 0.05）. A small amount of cells arranged disorder and vertebral body cell necrosis injured in hippocampus. Conclusion Chronic restraint stress can significantly impair spatial memory function in mice, the damage mechanism might be related to reduce the content ofhippocampus FGF2 and damege the hippocampal structure.

关 键 词：慢性束缚应激 学习记忆 小鼠 

分 类 号：R285[医药卫生—中药学]