高效液相色谱波长切换法同时测定微达康膏中的梓醇、刺五加苷B、刺五加苷E、淫羊藿苷和宝藿苷Ⅰ  

Simultaneous determination of catalpol,eleutheroside B,eleutheroside E,icariin,and baohuosideⅠin Weidakang paste by HPLC wavelength switching method

作　　者：张聪[1] 刘婕 解吉奕 

机构地区：[1]华中科技大学同济医学院附属协和医院药剂科,武汉430022 [2]湖北省中西医结合医院,武汉430015

出　　处：《中南药学》2017年第4期496-499,共4页Central South Pharmacy

摘　　要：目的建立HPLC波长切换法同时测定微达康膏中的梓醇、刺五加苷B、刺五加苷E、淫羊藿苷和宝藿苷Ⅰ的方法。方法采用Waters Nova-pak C_(18)色谱柱,以乙腈(A)-0.2%磷酸溶液(B)为流动相,梯度洗脱,流速0.9 mL·min^(-1)。结果梓醇、刺五加苷B、刺五加苷E、淫羊藿苷和宝藿苷Ⅰ分别在4.53~90.60μg·mL^(-1)、6.18~123.60μg·mL^(-1)、3.92~78.40μg·mL^(-1)、7.51~150.20μg·mL^(-1)、7.14~142.80μg·mL^(-1)与峰面积呈良好的线性关系,相关系数分别为0.9999、0.9998、1.000、0.9996、0.9999;平均加样回收率及相应的RSD分别为98.5%(1.2%)、98.9%(0.77%)、99.1%(0.90%)、97.5%(1.8%)、97.1%(0.84%)。结论本方法快速、准确度高、专属性好,可用于微达康膏的质量控制。Objective To develop an HPLC wavelength switching method to determine the content of catalpol, eleutheroside B, eleutheroside E, icariin, and baohuoside I in Weidakang paste simultaneously. Methods Waters Nova-pak Cls chromatographic column was adopted. The mobile phase was acetonitrile （A）-0.2% phosphoric acid solution （B） with gradient elution at 0.9 mL min-1. Results Catalpol, eleutheroside B, eleutheroside E, icariin, and baohuoside I showed a good linearity at 4.53 -- 90.60 μg mL-1 （r = 0.9999）, 6.18 -- 123.60 μg mL-1 （r -= 0.9998）, 3.92 -- 78.40 μg mL-1 （r = 1.000）, 7.51 -- 150.20 μg mL-1 （r = 0.9996）, and 7.14 -- 142.80 μg mL-1 （r = 0.9999）, respectively. The average recoveries and the corresponding RSD were 98.5% （1.2%）, 98.9% （0.77%）, 99.1% （0.90%）, 97.5% （1.8%）, and 97.1% （0.84%）. Conclusion The method is rapid, highly accurate and specific, which can be used in the quality control of Weidakang paste.

关 键 词：微达康膏 梓醇 刺五加苷B 刺五加苷E 淫羊藿苷 宝藿苷Ⅰ 波长切换法 

分 类 号：R917[医药卫生—药物分析学]