中和试验发现西安地区为肾综合征出血热混合型疫区  

Confirmation of Xi＇an as an epidemic zone of multiple hantavirus types by neutrolizing test

作　　者：王丽[1] 魏菁[1] 李慎[1] 马长安[2] 屈建惠 董建华[1] 马颖欣[3] 张全福[3] 李川[3] 王敬军[1] 余鹏博[1] 

机构地区：[1]陕西省疾病预防控制中心病毒病预防控制所,西安710054 [2]户县疾病预防控制中心,西安710300 [3]中国疾病预防控制中心病毒病预防控制所出血热室,北京102206

出　　处：《国际病毒学杂志》2017年第2期107-110,共4页International Journal of Virology

基　　金：2015年国家公益性行业科研专项项目（201502020）;陕西省疾病预防控制重大课题（2014A7）;陕西省社发攻关项目（2013K12-04-02）;陕西省卫生科研项目（2014-E1）

摘　　要：目的调查陕西西安地区汉坦病毒感染现状，分析当地肾综合征出血热（hemorrhagic fever with renal syndrome，HFRS）疫源地性质。方法根据HFRS发病报告数据，分析西安HFRS的发病特点。通过荧光定量RT—PCR方法，检测西安HFRS疫区家鼠和野鼠携带汉坦病毒情况及病毒型别。微量中和试验确定西安本地HFRS病人、HFRS疫苗接种者和隐性感染者血清中的汉坦病毒中和抗体水平并分型，回顾性调查阳性感染者。结果西安地区HFRS发病每年均有6—7月份的小高峰和10—12月份的大高峰；在当地642只家鼠和1546只野鼠肺组织中检出汉滩病毒RNA136份；143份人血清中123份检测到汉坦病毒中和抗体，未检测到中和抗体的有20份。中和抗体阳性者中判定为汉滩病毒感染者92人，占74．80％；判定为汉城病毒感染者3人，占2．44％；不能区分病毒感染型别者28人，占22．76％，3例感染汉城病毒者分别为HFRS病人、疫苗接种者和隐性感染者，调查显示3例均为本地感染。结论实验室和现场调查证实当地存在汉城型病毒本地感染，陕西西安地区是以汉滩病毒型为绝对优势的HFRS混合型疫区。Objective To analyze the status of Hantaan virus （HTNV） and Seoul virus （SEOV） infections in Xi＇an area, and to determine the natural foci of HFRS in Xi＇an, Shaanxi province. Methods The seasonal characteristics of HFRS in Xi＇an were analyzed according to the reported data. Hantavirus RNA from lung samples of mice and rats were analyzed and serotyped by quantitative real-time reverse transcription polymerase chain reaction （qRT-PCR）. The microneutralization tests were performed to detect Hantavirus types, and The protective antibodies against hantavirus were tested in the three groups, including HFRS patients, latent infections and vaccine recipients groups. Then a retrospective epidemiological investigation was carried out on the positive persons. Results There were two HFRS incidence peaks every year in Xi＇an, the small one was from June to July and the big one was from October to December. Of the lung samples from 642 mice and 1546 rats, 136 were Hantavirus RNA positive, and all of which belongs to Hantaan virus. Of 143 human sera, 123 were anti-hantavirus neutralizing antibody positive, and 20 were negative. Of these positive cases, 92 were diagnosed as infections of hantaan virus （74.80%）, 3 were diagnosed as infections of Seoul virus （2.44%）, and the remaining 28 cases could not be serotyped （22.76%）. Among the three cases of Seoul virus infection, one was HFRS patients, the second was HFRS vaccine recipient, and the third was latent infection. And retrospective survey indicated that all of the three cases were infected locally. Conclusions Laboratory and field investigation data confirmed that Seoul virus infection exists in Xi＇an area of Shaanxi province, where should be regarded as an epidemic area of multiple types of HFRS with hantaan virus as the predominant type.

关 键 词：汉滩病毒 汉城病毒 中和抗体 西安 疫区 

分 类 号：R512.8[医药卫生—内科学]