恩诺沙星及其代谢物在西伯利亚鲟体内的药动学及消除规律  

Pharmacokinetics and Elimination of Enrofloxacin and Ciproflowerxacin (Enrofloxacin Metabolite) in Acipenser baeri

作　　者：赵凤 周洲 刘永涛[2] 李小义 孔杰 陈飞雄 

机构地区：[1]贵州省水产研究所,贵州贵阳550025 [2]中国水产科学研究院长江水产研究所,农业部淡水鱼类种质监督检验测试中心,湖北武汉430223

出　　处：《西南农业学报》2017年第4期957-961,共5页Southwest China Journal of Agricultural Sciences

基　　金：贵州省科学技术基金项目“西伯利亚鲟气单胞菌性疾病模型的构建及药代动力学研究”[黔科合J字(2013)2190];贵州省农业攻关项目“鲟鱼精子超低温冷冻保存技术研究及应用”[黔科合NY(2015)3003-2];贵州省特色水产产业技术体系“疾病防控功能实验室”(GZCYTX2013-01102);贵州省农业科学院专项“贵州山区鲟鱼致病菌株的分离鉴定及药敏特性研究”[黔农科院院专项(2013)005]

摘　　要：【目的】为探明西伯利亚鲟体内恩诺沙星及其代谢产物环丙沙星的代谢动力学与消除规律,同时为科学合理使用恩诺沙星防治鲟鱼细菌性疾病和水产品的安全提供理论依据。【方法】采用高效液相色谱法分析20℃条件下进行恩诺沙星10 mg/kg体重口灌给药后西伯利亚鲟血液、肌肉和肝脏组织中恩诺沙星及代谢产物环丙沙星的浓度,药物浓度时间数据采用药代动力学软件3p97进行分析。【结果】西伯利亚鲟血液、肝脏和肌肉中恩诺沙星药物代谢动力学房室模型符合二房室模型,达峰时间(T_(max))分别为0.5、0.9和1.23 h;峰浓度值(C_(max))分别为0.329、4.162和3.350 mg/L;表观分布容积(V_d)分别为26.219、6.320和2.835 L/kg,吸收半衰期﹝T_((1/2)α)﹞分别为4.643、45.623和11.308 h;消除半衰期[T_((1/2)β)]分别为32.046、354.987和252.194 h。恩诺沙星代谢物环丙沙星在西伯利亚鲟血液、肝脏和肌肉中药物代谢动力学房室模型符合二房室模型,T_(max)分别为0.5、6.4和2 h;C_(max)分别为0.164、0.918和1.450 mg/L;Vd分别为43.760、7.678和0.122 L/kg;T_((1/2)α)分别为4.643、8.503和14.464 h;T_((1/2)β)分别为32.046、148.4和219.904 h。【结论】恩诺沙星其代谢产物环丙沙星在西伯利亚鲟体内药物代谢动力学房室模型符合二房室模型,其吸收、消除较缓慢。[ Method] The prent paper aims to discuss pbarmacokinetics and elimination of Enrofloxacin and Ciproflowerxacin in Acipenser baeri and provide the theoretical basis for scientific and rational utilization of Enrofloxacin against bacterial diseases of sturgeon and safety of aquatic products. [ Objectiwe ] The concentration of Enrofloxacin and Cipmflowerxacin in blood, muscle and liver of Acipenser baeri after orally enrofloxacin with dose of 10 mg/kg was determined by HPLC at 20 ℃ and the time data of drug concentration was analyzed by pharmacokinetics software 3p97. [ Result] The Enmfloxaein pharmacokinetics compartment modal in blood, liver and muscle ofAcipenser baeri accords with the two-compartment model and the Tmax, Cmmax, Vd, T（1/2） and T（1/2）β in blood, liver and muscle ofAcipenser baer/are 0.5 h, 0. 329 mg/ L, 26. 219 L/kg, 4. 643 and 32. 046, 0.9 h, 4. 162 mg/L, 6. 320 L/kg, 45. 623 h and 354. 987 h, and 1.23 h, 3. 350 mg/L, 2. 835 L/ kg, 11. 308 h and 252. 194 h, respectively. The ciproflowerxacin pharmacokinetics compartment model in blood, liver and muscle of Acipenser baeri accords with the two-compartment model also and the Tmax ,Cmax, Vd, T（1/2） and T（1/2）β in blood, liver and muscle of Acipenser baeri are 0.5 h, 0. 164 mg/L, 43. 760 L/kg, 4.643 h and 32.046 h, 6.4 h, 0.918 mg/L, 7.678 L/kg, 8.503 h and 148.4 h, and 2 h, 1.450 mg/L, 0. 122 L/kg, 14.464 h and 219. 904 h separately. [ Conclusion ] The enrofloxacin and ciproflowerxacin pharmacokinetics compartment model in tissues of Acipenser baeri accords with the two-compartment model and the absorption and elimination process in tissues of Acipenser baeri is slow.

关 键 词：恩诺沙星 环丙沙星 西伯利亚鲟 药动学 

分 类 号：S948[农业科学—水产养殖]