家蚕红色卵致死突变Fuyin-lre的性状特点与遗传分析  

Character Features and Genetic Analysis of Lethal Red Egg Mutant Fuyin-lre of Silkworm,Bombyx mori

作　　者：廖鹏飞[1] 丁善明[1] 陈安利[1] 李琼艳[1] 罗顺高[1] 朱水芬[1] 黄平[1] 董占鹏[1] 

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101

出　　处：《西南农业学报》2017年第4期969-974,F0003,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(31460640);云南省现代农业蚕桑产业技术体系建设项目(2015KJTX006);云南省重点新产品开发计划项目(2013BB007;2015BB008);国家现代农业产业技术体系专项(CARS-22)

摘　　要：【目的】为了解发现于云南家蚕种质资源"芙印"系统红色卵致死突变Fuyin-lre的性状特点和遗传性。【方法】观察Fuyinlre的卵形、卵色、胚胎发育及孵化情况,并与正常卵系统P50、突变红色卵标志系统re开展杂交、回交等遗传分析试验。【结果】Fuyin-lre系统出现全紫褐色卵、紫褐色卵与红色卵并存的2种蛾区,紫褐色卵能正常孵化发育,而红色卵稍透明,常会出现不规则的条状或块状的黑斑,胚胎发育至丙1时,停止发育不孵化,与已研究报道的红色卵突变re、ci、rec、b-4、ru等存在明显的差异而与rel相似;Fuyin-lre系统与P50杂交F_1代均为紫褐色卵,F_2代与BC_1代出现紫褐色卵∶红色卵为3∶1的突变蛾区,所有紫褐色卵能正常孵化发育而红色卵不孵化;Fuyin-lre系统与re的F_1、F_2和BC_1均会出现红色卵突变蛾区,依交配方式的不同,突变蛾区中有全红色卵、1/2红色卵和1/4红色卵等多种表现形式,其中红色卵存在正常孵化、孵化1/2或孵化3/4等差异。【结论】Fuyin-lre突变发生于re基因所在的第5染色体上,受隐性基因控制,纯合致死,遵循孟德尔遗传规律。[ Objective] The present study was conducted to investigate the character features and hereditary of red egg mutant Fuyin-lre which was found in silkworm （Bombyx mori） germplasm resource Fuyin. [ Method] We observed the egg shape, egg colour, embryo development and incubation of Fuyin-lre, and hybridizing with normal egg system P50, red egg mutant system to analyze the hereditary law. [ Resuh ] There were two kind batchs of Fuyin-lre system, all of the eggs were purple brown eggs in one batch, purple brown eggs and red eggs coexis- ted in the other batch. The purple brown eggs could develop normally, but the red eggs slightly transparence, often appeared irregular strips or blocks of dark spots, and the embryos of the red eggs stopped develop at bing-1 embryo. It was obviously different from the reported red egg mutant systems re,ci,re^c,b-4,ru, hut similar to rek Hybrid offspring （F1） of Fayin-lre and 1750 were purple brown eggs. The batchs which included purple brown eggs and red eggs （purple brown eggs： red eggs = 3：1 ） appeared in F2 and BC1. All the brown eggs hatched normally,but the red eggs could not hatch. The batehs included red eggs appeared in hybrid offspring F1 ,F2 and BC1 of Fuyin-lre and re. The red eggs in mutant batchs had various types, including all red eggs, 1/2 red eggs and 1/4 red eggs, and so on. Hatching rate of red eggs included normal hatching, hatching 1/2 or hatching 3/4 types. [ Conclusion] The Fuyin-lre mutant occurred in the fifth linkage group, followed recessive inheritance, and conformed to the mendelian inheritance.

关 键 词：家蚕 红色卵突变 致死 性状特点 遗传分析 

分 类 号：S851.347.31[农业科学—预防兽医学]