离子液体[C_(16)mim]Cl对泥鳅胚胎与仔鱼的毒性  

Study on the Toxicity Effect of [C_(16)mim]Cl to Misgurnus anguillicaudatus Embryos and Larvae

作　　者：南平[1] 燕帅国[1] 王亚星[1] 韩晓[1] 张岩晨 杜启艳[1] 常重杰[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《湖北农业科学》2017年第8期1518-1521,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(3071666);河南师范大学青年科学基金项目(5101049279082);河南师范大学青年骨干教师基金项目(5101049470609)

摘　　要：为了研究离子液体[C_(16)mim]Cl的生物毒性作用,以泥鳅(Misgurnus anguillicaudatus Cantor)胚胎和仔鱼为受试动物,分别以胚胎发育的囊胚期、原肠胚期、神经胚期和尾芽期4个发育时期以及孵出期和孵出3 d的仔鱼作为处理的起始点进行急性毒性试验。结果显示,随着[C_(16)mim]Cl浓度的增加和处理时间的延长,泥鳅胚胎的孵化率逐渐下降,畸形率则在逐步升高;其中,刚孵出的仔鱼24 h与48 h的半数致死浓度(LC_(50))及安全浓度(SC)分别为0.555、0.453、0.091 mg/L;孵出3 d的仔鱼24 h与48 h的LC_(50)及SC分别为0.444、0.273、0.031 mg/L。表明在较低剂量内,离子液体[C_(16)mim]Cl对泥鳅胚胎及其仔鱼的发育表现出明显的致畸与致死作用,并且该毒性作用具有显著的剂量-效应关系。The toxic effect of chloride-l-sixteen alkyl-3-methylimidazolium chinook（[C16nim]Cl） to the embryos and larvae hi＂ Misgurnus anguillicaudatus Cantor was studied. The M. anguillicaudatus were exposed to [C16mim]Cl from the beginning of different stages including the embryonic development of blastoderm, mid-gastrula, neurula and tail bud stage, and the larval of hatching period and 3 days after hatching. The results showed that the hatching rate of M. anguiUicaudatus embryos decreased and the abnormality of M. anguillicaudatus embryos increased with the increase of [C16nim]Cl treatment time and treatment concentration; and a significant dose-response relationship was observed. The 24 h-LCso, 48 h-LC50 and SC of the newly hatched larvae were 0.555,0.453 and 0.091 mg/L respectively; whereas, the 24 h-LC50, 48 h-LC50 and SC of the 3-day-old larvae were 0.444, 0.273 and 0.031 mg/L. The above results showed that [C16mim]Cl had obvious teratogenic and lethal effect to the embryos and larvae of M. anguillicaudatus.

关 键 词：泥鳅(Misgurnus anguillicaudatus Cantor) 胚胎发育 仔鱼 [C16mim]Cl 毒性 

分 类 号：S966.4[农业科学—水产养殖] X52[农业科学—水产科学]