大鼠腹主动脉瘤中早期生长反应因子1mRNA表达水平的研究  

Expression of early growth response factor-1 mRNA in rat abdominal aortic aneurysm( AAA) pathogenesis

作　　者：董海鹏[1] 王石[1] 刘程伟[1] 徐桂超[1] 金松[1] 

机构地区：[1]佳木斯大学附属第一医院血管外科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2017年第2期1-2,5,共3页Heilongjiang Medicine and Pharmacy

基　　金：国家自然科学基金;编号:30801123;81070254;教育部留学回国人员科研启动基金;编号:教外司留【2014】1685号

摘　　要：目的:检测早期生长反应因子-1(Egr-1)mRNA在大鼠腹主动脉瘤(AAA)模型组织中的表达情况,以探讨其在腹主动脉瘤发病机制中的作用。方法:20只SD雄性大鼠,随机分成2组,一组为对照组,另一组为实验组,实验组为弹力蛋白酶灌注(20U/m L)1m L,持续30min。分别术前,术后14d测量腹主动脉直径。应用q-PCR方法检测动脉瘤中早期生长反应因子-1 mRNA的表达,数据均以x±s或者配对t检验进行两组之间的差异分析。结果:实验组动物模型均已达到腹主动脉瘤诊断标准(对照组:1.400±0.033,实照组:2.950±0.171,每组10只,P<0.05)。与对照组相比,实验组Egr-1mRNA的表达明显高于对照组(对照组:1.026±0.122,实验组:6.177±0.721,P<0.05)。结论:早期生长反应因子-1 mRNA在大鼠腹主动脉瘤中的表达明显高于对照组,说明早期生长反应因子-1可能在介导腹主动脉瘤的形成中发挥重要作用。Objective： To investigate the relationship between early growth response factor - 1 （ Egr - 1 ） and the formation of abdominal aortic aneurysm （AAA） in rats. Methods ：20 SD male rats were randomly divided into two groups： the control group and the experimental group. The experimental group was injected with elastase （20U/mL） lml for 30min. Aortic diameter was measured before and after performance of elastase perfusion and before they were killed at 14th Day. The change of Egr - 1 mRNA was detected by q - PCR. The data were analyzed with x ± s or paired t test for the difference between the two groups. Results： The animal model of experimental group had accorded with the standard of diagnosis of abdominal aortic aneurysm （ control group ： 1. 400 ±0. 033 ; the control group： 2.950 ±0. 171, each group of 10, P 〈 0. 05）. Compared with the control group, the expression of Egr - 1 mRNA in the experimental group was significantly higher than that in the control group （ control group ： 1. 026 ± 0. 122, the experimental group ： 6. 177 ± 0.721, P 〈 0. 05 ）. Conclusion： The changing of Egr - 1 mRNA expression is parallel to AAA pathogenesis. It suggested that Egr- 1 may affect on AAA formation.

关 键 词：早期生长反应因子-1 腹主动脉瘤 Q-PCR 

分 类 号：R654.3[医药卫生—外科学]