IL-4、IL-10及IL-12与乙型肝炎病毒感染进展的研究  

Investigated of influence IL-4,IL-10 and IL-12 on the progress of hepatitis-B-virus infection

作　　者：唐萍[1] 苏泽红[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南衡阳421001

出　　处：《微量元素与健康研究》2017年第3期72-75,共4页Studies of Trace Elements and Health

摘　　要：目的:通过比较慢性HBV感染组与HBV感染自行恢复组间血清细胞因子水平差异,及其与HBsAg、HBsAb水平之间相关性分析,探讨细胞因子对HBV感染进展的影响。方法:使用ELISA方法定量检测慢性HBV感染组82例患者及HBV感染自行恢复组65例患者血清中HBsAg、HBsAb、HBeAg、HBeAb、HBcAb及细胞因子IL-4、IL-10、IL-12的水平。并对各组细胞因子水平及其与HBsAg、HBsAb水平进行相关性分析。结果:IL-12、IL-10水平在慢性HBV感染组中明显高于HBV感染自行恢复组(P=0.042,P=0.029),而IL-4水平显著低于HBV感染自行恢复组(P=0.001)。各组血清中的IL-12水平与IL-4水平都呈负相关(R=-0.441,P<0.001;R=-0.462,P<0.001);IL-12水平在慢性HBV感染组血清中与HBsAg水平呈负相关(R=-0.241,P=0.029),在HBV感染自行恢复组血清中与HBsAb水平呈正相关(R=0.346,P=0.05);而IL-4水平在慢性HBV感染组血清中与HBsAg不相关(P>0.05),与HBsAb水平呈负相关(R=-0.348,P=0.04)。IL-10水平在两组间没有相关性(P>0.05)。结论:血清中IL-12、IL-4、IL-10的分泌紊乱可能是导致慢性HBV感染的原因之一,且慢性HBV感染组中存在异常免疫应答;血清IL-4与IL-12水平的协调分泌对HBV的转归有重要作用。Objective：By comparing the levels of serum cytokine levels in patients with chronic HBV infection and HBV infection, and the correlation of level of serum between various cytokine and cytokine with HBsAb , HBsAg was analyzed. And investigated the effects of cytokines on the progression of HBV infection. Methos： Used enzyme - linked immunosorbent assay（ELISA） quantitative to detected level of HBsAg, HBsAb, HBeAg, HBeAb, HBcAb and IL - 4, IL - 10, IL - 12 in serum of 82 patients with chronic I-tBV infection and 65 patients with HBV infection spontaneous recovery. And analysised the correlation of level of serum between various cytokine and cytokine with HBsAb , HBsAg. Results： The Level of serum IL- 10 and IL- 12 in Chronic HBV infection group are higher than in HBV infection spontaneous recovery group （ P = 0.042, P = 0.029 respectively） ; But levels of serum IL - 4 in Chronic I-tBV infection group are lower than in HBV infection spontaneous recovery group （P = 0. 001）. Level of serum IL- 12 with HBsAg in chronic HBV infection was showing negative correlation（R = - 0.241, P = 0.029） ; level of serum IL - 12 and IL - 4 what ever in chronic HBV infection group or HBV infection spontaneous recovery group Both showing negative correlation （ R = - 0.441, P 〈 0.001 ; R = - 0.462, P 〈 0. 001, respectively）. In addition , level of serum IL - 4 with HBsAb in HBV infection spontaneous recovery group was showing negative correlation （R = -0.348, P = 0.04）, and level of serum IL- 12 with HBsAb was showing positive correlation in HBV infection spontaneous recovery group（ R = 0. 346, P = 0.05 ）. They are was no correlation that level of serum IL - 10 with IL - 4 , IL - 12, HBsAg and HBsAb in each group. Conclusion：The paraseeretion of IL - 4, IL- 10 and IL- 12 in serum may be one of the causes of chronic HBV infection, and in chronic HBV infection group has a abnormal immune response. Further more, the coordinated secretion of serum IL - 4 and IL - 12 levels has an important role in the o

关 键 词：HBV感染 IL-4 IL-12 IL-10 HBV感染进展 

分 类 号：R512.62[医药卫生—内科学]