金黄色葡萄球菌对奶牛乳腺成纤维细胞α-SMA及collagen-Ⅰ表达的影响  

Effect of Staphylococcus aureus on the expression ofα-SMA and collagen-Ⅰin bovine mammary fibroblasts

作　　者：杨彬[1] 孙志鹏[1] 徐丹丹[1] 赵佳琦[1] 武瑞[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2017年第5期828-832,共5页Chinese Journal of Veterinary Science

基　　金：黑龙江八一农垦大学博士后启动基金(XYB2013-11)

摘　　要：为研究金黄色葡萄球菌(S.aureus)对奶牛乳腺成纤维细胞(BMFB)α-SMA及collagen-Ⅰ表达的影响,本研究分别用不同浓度的热灭活金黄色葡萄球菌(0,10~4,10~5,10~6,10~7和10~8 CFU/mL)刺激BMFB,24h后采用Real timePCR方法、Western blot方法及免疫荧光法检测α-SMA及collagen-ⅠmRNA和蛋白的表达量。结果显示,不同浓度灭活的金黄色葡萄球菌处理细胞的α-SMA及collagen-Ⅰ均能显著升高(P<0.05),其中以10~5 CFU/mL刺激组的α-SMA及collagen-Ⅰ的mRNA和蛋白的表达量最高。结果表明,金黄色葡萄球菌可以使奶牛乳腺成纤维细胞转分化为肌成纤维细胞,为揭示金黄色葡萄球菌性乳腺炎发生硬化的机理提供数据。In order to investigate the effect of Staphylococcus aureus （S. aureus ） on the expression of α-SMA and collagen- I in bovine mammary fibroblasts （BMFB）. BMFB were stimulated with heat-killed S. aureus （HKSA） at 104 , 105 , 106 , 107 , 108 CFU/mL. The expression of α-SMA and collagen- I was detected by real time-PCR,Western blot and immunofluorescence after 24 h. The results showed that the expression level of α-SMA and eollagen-I increased significantly after 24 h stimulation compared with unstimulated BMFB （P 〈0.05）. The expression of α-SMA and collagen I reached the maximal level at 105 CFU/mL. The results indicated that heat-killed S. au- reus could induce differentiation of BMFB into myofibroblasts.

关 键 词：金黄色葡萄球菌 奶牛乳腺成纤维细胞 Α-SMA collagen-Ⅰ 

分 类 号：S858.23[农业科学—临床兽医学]