莲壳膳食纤维微波-双酶协同提取工艺优化  

Optimization on microwave-two enzymes synergistic methods for dietary fiber extraction in lotus seed shell

作　　者：陈铁壁[1,2] 靳娜[2] 王建辉[2] 刘冬敏[1,2] 樊敏[1] 邓胜国[1] 陈颖奕 

机构地区：[1]湖南科技学院化学与生物工程学院,湖南永州425199 [2]长沙理工大学湖南省水生资源食品加工工程技术研究中心,湖南长沙410114

出　　处：《食品与机械》2017年第3期164-167,共4页Food and Machinery

基　　金：国家自然科学基金青年科学基金项目(编号:31301564);湖南省自然科学基金项目(编号:2015JJ2011);湖湘青年英才支持计划项目(编号:2015RS4051);永州市科技局科技计划项目(编号:永科发[2009]20号);湖南省高校科技创新团队支持计划资助(编号:2012-318);湖南省水生资源食品加工工程技术研究中心开放基金资助项目(编号:2016GCZX02;2015GCZX08)

摘　　要：以莲子壳为原料,采用微波-双酶协同提取莲壳膳食纤维,在单因素试验结果的基础上,进行正交试验,研究莲子壳筛目数、料液比、木瓜蛋白酶和糖化酶用量及提取时间对莲壳膳食纤维提取工艺的影响。结果表明:其最佳提取工艺条件为莲壳筛目数60目,液料比201(mL/g),木瓜蛋白酶加入量3%,微波协同木瓜蛋白酶酶解提取时间为6×20s(提取6次,每次20s,间隔2min),糖化酶用量4%,微波协同糖化酶提取时间8×20s(提取8次,每次20s,间隔2min),该条件下莲壳膳食纤维提取率为72.36%。与双酶提取法相比,微波-双酶协同提取法可缩短70%的时间,提取率提高82.87%。To investigate the effect of microwave-two enzymes synergistic extraction methods on dietary fiber yields in lotus shell,the sieving mesh number of the lotus seed shell,ratio of solid-liquid,papain and glucoamylase dosages and extraction time were set out to conduct the orthogonal tests based on the results of single factor experiments.The optimal extraction conditions were as followed：sieving mesh of 60,ratio of liquid-solid 201,papain dosage 3%,papain extraction time of 6×20s（extracting 6times,each time for20 swith intervals of 2min）,dosage of glucoamylase 4%,and glucoamylase extraction time 8×20s（extracting 8times,each time for20 swith intervals of 2min）.Under the optimal conditions,the extraction rate of dietary fiber in lotus shell was 72.36%.Compared with enzyme extraction,microwave-two enzymes synergistic extraction can save 70%time,and greatly improve the extraction rate over82.87%than two enzyme method.

关 键 词：莲子壳 膳食纤维 微波 木瓜蛋白酶 糖化酶 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]