种植与根植栽培丹参含量比较研究  

Comparative Study on the Content of Salvia miltiorrhiza Bunge Planted with Seedling Cultivation and Root

作　　者：于凡[1,2,3] 李国转 陈卫东[1,2,4] 王国凯[1,2,4] 彭代银[1,2,4] 俞年军[1,2,4] 李勇 YU Fan LI Guozhuan CHEN Weidong WANG Guokai PENG Daiyin YU Nianjun LI Yong(School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China Syergetic Innovation Center of Anhui Authentic Chinese Medicine Quality, Hefei 230012, China Institute of Traditional Chinese Medicine Resources Protection and Development, Anhui Academy of Chinese Medicine, Hefei 230012, China Spring Wei Anhui Agricultural Science and Technology Co, Ltd. Taihe 236626, China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽道地中药材品质提升协同创新中心,安徽合肥230012 [3]安徽春之蔚农业科技有限公司,安徽太和236626 [4]安徽省中医药科学院中药资源保护与开发研究所,安徽合肥230012

出　　处：《云南中医学院学报》2017年第2期81-84,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：安徽道地中药材品质提升协同创新中心项目(2011协同创新中心);中医药行业科研专项安徽种植种苗基地建设项目(201207002);"2016-2018"年安徽高校科研平台创新团队:现代中药质量控制研究项目;安徽省重点研究与开发计划项目(1704a0802145)

摘　　要：目的比较种植、根植不同方法栽培而成的丹参不同部位化学成分的含量,用以选出合理的栽培方式。方法根据2015版《中国药典》中记载的方法采用高效液相色谱法(HPLC),对种植、根植两种不同栽培方式培育出来的丹参中的根、茎、叶、花托、须根5个部位进行丹酚酸B、丹参酮类(丹参酮IIA、隐丹参酮、丹参酮I)的含量测定。结果丹参的不同部位含量有显著差异,两类丹参中丹参酮类与丹酚酸B在不同部位分布的含量均依次为主根>须根>花托>茎>叶;根植丹参的主根及茎中所含丹参酮类高于种植丹参,就丹酚酸B而言,种植丹参5个部位中丹酚酸B的含量均高于根植丹参。结论种植丹参中丹酚酸B含量高于根植丹参,根植丹参的主根中丹参酮类比种植丹参含量高;可根据药材所需成分对丹参栽培方法进行合理的选择,指导丹参规范合理种植。Objective To compare the contents of different parts of Salvia milfiorrhiza in different cultivation methods and to select the reasonable cultivation mode. Methods According to the method described in the Chinese Pharmacopoeia 2015 Edition, using high performance liquid chromatography （HPLC） to determinate the content of salvianolic acid B and tanshinone （Salvia miltiorrhiza of tanshinone IIA, cryptotanshinone, tanshinone I ） which come from growning on different latitude in different breeding cultivation, rooted in two different planting Danshen root, stems, leaves, receptacle, fibrous roots. Results There were significant differences in different parts of Salvia miltiorrhiza content, plant Salvia miltiorrhiza and rooted salvia mihiorrhiza, tanshinones and salvianohc acid B in different parts of the distribution of content are root 〉 fibrous roots 〉 receptacle 〉 stem 〉 leaf; tanstfinone content in root and stem rooted in Salvia miltiorrhiza was higher than that of plant Salvia miltiorrhiza. The content of salvianolic acid B in plant Salvia miltiorrhiza of five parts were higher than the content in rooted salvia miltiorrhiza. Conclusion Salvianolic acid B in plant Salvia miltiorrhiza was higher than that in rooted salvia miltiorrhiza, but tanshinone was lower in rooted. Salvia miltiorrhiza cultivation methods can be a reasonable choice depending on the desired composition, guide rational planting.

关 键 词：丹参 不同部位 含量测定 丹酚酸B 丹参酮类 

分 类 号：R284.2[医药卫生—中药学]