“桂枝与白芍”药对不同比例配伍的HPLC指纹图谱探讨  

Discussion on HPLC fingerprints for the different compatibility proportion Ramulus Cinnamomi and Radix Paeoniae Alba

作　　者：陈永财 钱江辉 王彬辉[4] 沙先谊[2,3] CHEN Yongcai QIAN Jianghuiz WANG Binhui SHA Xianyi(Department of Pharmacy, Wenzhou Hospital of Traditional Chinese Medicine, Zhejiang Provicne, Wenzhou 325000, China Staff Room of Pharmacy, School of Pharmacy, Fudan University, Shanghai 201203, China Academy of In- tegrative Chinese and Western Medicine, Fudan University, Shanghai 201203, China Department of Pharmacy, Af- filiated Hospital of Medical College of Taizhou University, Zhejiang Province, Taizhou 318002, China)

机构地区：[1]浙江省温州市中医院药剂科,浙江温州325000 [2]复旦大学药学院药剂学教研室,上海201203 [3]复旦大学中西医结合研究院,上海201203 [4]台州学院医学院附属市立医院药剂科,浙江台州318002

出　　处：《中国医药导报》2017年第13期29-33,64,共6页China Medical Herald

基　　金：浙江省中医药科技计划项目(2015ZA195);浙江省温州市公益性科技计划项目(Y20140746)

摘　　要：目的建立"桂枝与白芍"药对不同比例配伍的高效液相色谱(HPLC)指纹图谱,探讨桂枝与白芍不同比例配伍后化学成分差异性及其煎出率变化。方法采用HPLC法,以Dikma Spursil C18(250 mm×4.6 mm,5μm)为色谱柱,流动相乙腈-0.1%冰醋酸水洗脱,温度25℃,进样量10μL,检测波长254 nm,流速1 m L/min。对"桂枝与白芍"药对(1∶1)、(1∶2)、(5∶3)不同比例配伍的HPLC指纹图谱采用相似度评价软件(2004A版)分析,并用各药对共有色谱峰面积与单煎相对应色谱峰面积的比值比较分析。结果确定18个共有峰,指认其中6个色谱峰,对共有峰归属,并进行相似度评价。初步确立单味白芍及桂枝白芍(1∶1)、(1∶2)、(5∶3)配伍后白芍中共有峰各成分煎出率依次提高,桂枝白芍(5∶3)时煎出效果最佳;桂枝白芍(5∶3)、(1∶2)配伍肉桂酸煎出率降低,可桂枝白芍(1∶1)配伍后肉桂酸煎出率接近单味桂枝,煎出效果最佳。结论该方法简单、准确、快速,重复性好,能有效地对"桂枝与白芍"药对的质量控制和成分鉴别及配伍理论提供依据。Objective The high performance liquid chromatography （HPLC） fingerprints for the different compatibility proportion Ramulus Cirmamomi and Radix Paeonioe Alba was developed to explore the component difference and the changing of solution rate. Methods The HPLC method with Dikma Spursil C18（250mm×4.6mm,5μm） column was used, the mobile phase was acetonitrile-0.1% acetic acid solution with gradient elution, the column temperature was 25℃, the inject volume was 10 μL, the detection wavelength was set at 254 nm, the flow rate was 1 mL/min. Similarity evaluation software （2004A edition） was adopted to analyze the HPLC fingerprints of the different compatibility propor- tion of Ramulus Cinnamomi and Radix Paeoniae Alba at the ratio of 1：1, 1：2 and 5：3, and the common peaks area were compared with the single drug solution. Results 18 common peaks were found and 6 chromatographic peaks were iden- tified. Evaluation of similarity was done for these common peaks. The results showed that the extraction rate of common peaks from Radix Paeoniae Alba increased in the order of 1：1, 1：2, 5：3 for the mixture of Ramulus Cinnamomi and Radix Paeoniae Alba, and reached its highest level at 5：3. While the extraction rate of cinnamic acid decreased in the order of 5：3, 1：2 and reached the lowest level at a ratio of 1：1, which was equal with the extraction rate of a single Ra- mulus Cinnamomi. Conclusion The method is simple, reproducible, and accurate, which can be applied to the qualitycontrol and provides some compatibility theoretical bases for the drug pair of Ramulus Cinnamomi and Radix Paeoniae A lba.

关 键 词：桂枝 白芍 药对 高效液相色谱 指纹图谱 成分归属 

分 类 号：R284[医药卫生—中药学]