白藜芦醇对牛精液冷冻保存的抗氧化作用研究  

Anti-oxidation of Resveratrolon Cryopreservation of Bovine Semen

作　　者：刘程[1,2] 方乾[1] 杨瑞[1] 马国际 李梅 王艳华 胡建宏[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]甘肃省天水市秦州区工业和信息化局 [3]陕西省家畜改良站 [4]白水县家畜改良站 [5]拉萨市动物疫病预防控制中心

出　　处：《畜牧兽医杂志》2017年第3期4-6,共3页Journal of Animal Science and Veterinary Medicine

基　　金：陕西省农业科技创新转化项目(NYKJ-2016-17);西北农林科技大学大学生科技创新项目

摘　　要：为了研究白藜芦醇对牛精液冷冻保存效果的影响,本试验在牛精液冷冻稀释液中添加白藜芦醇,测定冷冻-解冻后精子的总抗氧化能力、丙二醛含量和谷胱甘肽过氧化物酶活力。结果表明,随着白藜芦醇浓度的升高(15~150μM),精子T-AOC和GSH-Px活力逐渐增强,MDA含量降低。稀释液中添加150μM白藜芦醇时,精子GSH-Px活力和MDA含量分别达到113.3μmol/L和96.14nmol/mL,显著高于对照组(P<0.05);100μM组精子T-AOC最高,但与150μM组差异不显著(P>0.05);白藜芦醇浓度为200μM时,T-AOC及MDA含量显著低于对照组(P<0.05),但GSH-Px活力与对照组差异不显著(P>0.05)。This study was aimed at investigated the cryopreservation ef{ects of resveratrol on bovine semen, different con- centrations of resveratrol were added intofrozen diluent, and antioxidant indexes including total antioxidant capacity （T-AOC）, malondialdehyde （MDA） content and glutathione peroxidase （GSH-Px） activity were measured. The results showed that with the increase of resveratrol concentration （15 tIM-150 μM）, the T-AOC and GSH-Px activity of bovine sperm increased while MDA content decreased gradually. The GSH-Px activity and MDA content in group added 150 btMresveratrol were 113.3 mol/ 1 and 96.14 nmol/ml, respectively, and were remarkably higher than that in control group （P〈0.05）. Besides, T-AOC of 100 μM group was the highest, but no significant difference were observed compared to 150μM group （P〉0.05）. T-AOC and MDA content of the group added 200 μM resveratrol were significantly lower than those in control group, while theno differ- ence o{ GSH-Px activity between the two groups were found （P〉 0.05）.

关 键 词：白藜芦醇 牛精液 冷冻保存 抗氧化性能 

分 类 号：S814.8[农业科学—畜牧学]