华支睾吸虫硫氧还蛋白过氧化物酶对脓毒症小鼠的保护作用  

Protective effect of recombinant Clonorchis sinensis TPx on LPS-induced sepsis mice

作　　者：尹红玲[1,2] 蒋娟[1,2] 黄怀球[3] 胡旭初[1,2] 

机构地区：[1]中山大学中山医学院寄生虫学教研室,广东广州510080 [2]中山大学中山医学院热带病防治研究教育部重点实验室,广东广州510080 [3]中山大学附属第三医院皮肤科,广东广州510630

出　　处：《热带医学杂志》2017年第4期421-423,429,F0003,共5页Journal of Tropical Medicine

基　　金：国家重点基础研究发展计划(973计划)(2010CB530000)

摘　　要：目的探讨华支睾吸虫(Cs)硫氧还蛋白过氧化物酶(TPx)对脂多糖(LPS)致脓毒症小鼠的保护作用。方法 80只Balb/c雄性小鼠随机分成两组,腹腔注射LPS(4 mg/kg)制备脓毒症小鼠模型,造模后30 min尾静脉分别注射PBS、rCsTPx(2.5 mg/kg),观察注射后各组小鼠72 h死亡率;重复实验,每组分别于实验后的3、6、9、12、24、48、72 h"安乐死"处死小鼠,收集血清并取24 h组织用于病理切片观察。结果尾静脉注射CsTPx治疗的小鼠生存率由10%提高到40%;检测血清谷草转氨酶(AST)和谷丙转氨酶(ALT)水平在造模后6、9、12、24 h明显下降,并且血清中炎症因子IL-6在3、6、9 h也明显下调,差异均有统计学意义;组织HE染色揭示CsTPx治疗组小鼠肾组织损伤减轻,炎症细胞浸润也减少。结论 rCsTPx对LPS致脓毒症小鼠有较好的保护作用。Objective To study the protective effects of recombinant Clonorchis sinensis TPx on LPS-induced sepsis mice. Methods The total of 80 male Balb/c mice were injected intraperitoneally （i.p.） with 4 mg/kg of LPS and randomly divided into two groups. The control group was injected with phosphate buffer saline and the treatment group was injected thioredoxin peroxidase （2.5 mg/kg ） via the caudal vein 30 min after LPS injection. Their mortality of 72 hour were observed. The specimen was collected after the mice were killed at 3, 6, 9, 12, 24, 48 and 72 h. The AST and ALT levels, cytokine and histological evaluation were investigated. Results Thioredoxin peroxidase （TPx） of Clonorchis sinensis increased the surviv- al rates of mice from 10% to 40%. In addition, there were significant decreased on AST and ALT levels at 6, 9, 12 and 24 h ; down-regulation of IL-6 levels at 3, 6 and 9 h were observed in these mice. Tissues HE staining revealed that CsTPx treatment reduced renal injury and inflammatory cell infiltration. Conclusion The recombinant thioredoxin peroxidase （TPx） could have a favorable effect on LPS-induced sepsis mice.

关 键 词：华支睾吸虫 硫氧还蛋白过氧化物酶 脓毒症 脂多糖 

分 类 号：R383.22[医药卫生—医学寄生虫学]