硫化氢对肠缺血-再灌注损伤大鼠炎症因子及内毒素血症的影响  

Role of hydrogen sulfide in endotoxinemia and inflammation in rats with intestinal ischemic-reperfusion injury

作　　者：吴忠明[1] 卢根林[1] 吴海明[1] 吴爱兵[2] 王宏宾[3] 

机构地区：[1]义乌市中心医院普通外一科,浙江义乌322000 [2]广东医学院附属医院肿瘤中心,广东湛江523808 [3]青海大学附属医院肝胆胰外科,青海西宁810001

出　　处：《中华全科医学》2017年第6期921-923,共3页Chinese Journal of General Practice

基　　金：国家自然科学基金青年科学基金项目(81201672);浙江省义乌市人才引进立项项目(2012-R-04)

摘　　要：目的研究硫化氢对肠缺血-再灌注损伤大鼠血C反应蛋白、降钙素原、内毒素、肿瘤坏死因子-α、白介素-6、白介素-8的影响。方法将64只雄性Wistar大鼠随机分为A(假手术)组、B(缺血-再灌注)组,C(缺血-再灌注+NaH S)组,D[缺血-再灌注+炔丙基甘氨酸(PPG)]组。经腹正中切口剖腹及游离肠系膜上动脉(SMA),钳夹B、C、D组大鼠SMA 60 min和再灌注120 min,建立大鼠肠缺血-再灌注损伤模型。C组在灌注前10 min向大鼠尾静脉注入100μmol/kg NaH S后按1 mg/(kg·h)输注直到再灌注2 h,D组在缺血前10 min向腹腔注入PPG50 mg/kg。检测血CRP、降钙素原、内毒素、H_2S、TNF-α、IL-6、IL-8并进行血培养,测定回肠组织匀浆中H_2S。结果与A组比较,B组的CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8升高,H_2S降低。与B组比较,C组的CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8降低,H_2S升高。与C组比较,D组CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8升高,H_2S降低(P<0.01)。B、C、D组分别有14、6、15例血标本培养出大肠杆菌。H_2S与CRP、降钙素原、内毒素、TNF-α、IL-6、IL-8呈负相关。结论 H_2S降低肠缺血-再灌注损伤大鼠血炎症因子,改善内毒素血症。Objective To investigate the influence of hydrogen sulfide on the levels of plasma C reactive protein（ CRP）,procalcitonin（ PCT）,endotoxin,TNF-α,IL-6 and IL-8 in rats with intestinal ischemic-reperfusion injury. Methods A total of 64 healthy male Wistar rats were randomly divided into sham operation group（ A）,ischemia-reperfusion group（ B）,ischemic-reperfusion and sodium hydrosulfide group（ C）,and ischemia-reperfusion and PPG group（ D）,n = 16. The intestinal ischemic-reperfusion injury rat model was prepared as follow： used a midline incision for laparotomy,freed and clamped the superior mesenteric artery（ SMF） for 60 min,and then reperfusion for 120 min. The tail vein injection of sodium hydrosulfide[100 μmol/kg] was performed 10 min prior to the onset of reperfusion,and continuous infusion[1 mg/（ kg·h） ]until reperfusion for 2 h in group C. The intraperitoneal injection of PPG（ 50 mg/kg） was performed in group D 10 min before the ischemia. The levels of CRP,PCT,endotoxin,H_2S,TNF-α,IL-6 and 8 were tested; the synchronic blood culture was performed to evaluate the endotoxemia. H_2S in the ileal homogenate was also measured. Results Compared with group A,the level of plasma endotoxin,PCT,CRP,TNF-α,IL-6 and 8 in the group B increased but H_2S decreased. Compared with group B,the level of plasma endotoxin,PCT,CRP,TNF-α,IL-6 and 8 in group C decreased. Compared with C group,the level of plasma endotoxin,PCT,CRP,TNF-α,IL-6 and 8 in the group D increased. The level of plasma H_2S in group D was significantly lower than that in group B and C. There were 14,6 and 15 blood samples respectively in group B,C and D infected by Escherichia colic. H_2S negatively correlated with the levels of CRP,CT,endotoxin,TNF-α,IL-6 and IL-8. Conclusion H_2S can decrease the level of inflammatory factor（ PCT,CRP,TNF-α,IL-6 and IL-8） and attenuates endotoxinemia in rats with intestinal ischemic-reperfusion injury.

关 键 词：缺血-再灌注 硫化氢 内毒素 炎症因子 

分 类 号：R333.3[医药卫生—人体生理学] R364.12[医药卫生—基础医学]