两种生态型拟南芥的室内培养  

Indoor culture techniques for Arabidopsis thaliana of two ecotypes

作　　者：卞金鸽 王培正[1] 刘彤[2] 魏凯璐 张婵[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]石河子大学生命科学学院,新疆石河子832003

出　　处：《河南科技学院学报（自然科学版）》2017年第2期8-10,14,共4页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：河南省高等学校重点科研项目计划(15A180048);河南师范大学博士启动课题(qd14169)

摘　　要：以拟南芥模式种哥伦比亚生态型和中国分布的新疆生态型为研究材料,探索两种生态型拟南芥在人工气候箱内培养的技术方法.结果表明:通过MS培养基法与土培法相结合,可以培养出长势较好的拟南芥植株.哥伦比亚生态型和新疆生态型拟南芥种子的萌发势分别为(72.24±17.30)%和(77.62±7.36)%,萌发率分别为(81.13±5.98)%和(81.90±1.56)%,且幼苗存活率均达到了95%以上.研究结果可为科研工作者在相似条件下进行拟南芥培养提供借鉴.Columbia ecotype and Chinese Xinjiang ecotype were used to explore the technology of the cultivation of Arabidopsis thaliana in artificial climate chamber.The results showed that the combination of the MS culture medium method and soil culture method provided an appropriate indoor conditions for growth and reproduction of A. thaliana.The germination potentials of Columbia ecotype and Xinjiang ecotype were（72.24±17.30）% and（77.62±7.36）%,respectively;and the germination rates were（81.13±5.98）% and（81.90±1.56）%,respectively.In addition,the survival rates of the seedlings of two ecotypes were both more than 95%.The results of this paper provide a reference for scientific research workers under similar conditions.

关 键 词：拟南芥 移栽法 萌发势 萌发率 

分 类 号：Q94-331[生物学—植物学]