不同添加物对拟康木霉St-zn-9在土壤浸渍液中生长的影响  

Effects of adding different additive on the growth of Trichoderma pseudokoningii strain St-zn-9 on soil-impregnantmedia

作　　者：冯振宇[1] 杨蕊[1] 霍云凤[1] 郎剑锋[1] 石明旺[1] 

机构地区：[1]河南科技学院资源与环境学院,河南新乡453003

出　　处：《河南科技学院学报（自然科学版）》2017年第2期11-14,共4页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金："十二五"国家科技支撑计划项目(2015BAD26B01);河南省教育厅高等学校重点科研项目(17A210006);国家级大学生创新训练项目(2015CX052)

摘　　要：为明确添加物对木霉打破土壤抑菌能力的影响,以土壤浸渍液培养基为基础培养基,研究了不同添加物添加后木霉菌丝干质量和产孢量的变化.结果表明:不同添加物对木霉菌丝干质量和产孢量的影响不同,其中添加枣果煎汁、葡萄糖、尿素、磷酸二氢钾、马铃薯煎汁和玉米秸秆煎汁时菌丝干质量与产孢量均显著高于对照.组合培养以组合D(加入玉米秸秆煎汁和尿素)效果最佳,产孢量达到(14.17±0.38)×10~7个/mL.To determine the ability of additive to break the soil fungistasisto Trichoderma pseudokoningii St-zn-9,using the soil-impregnant media as the basic culture medium,the effect of add different additive nutrients to the mycelium dry weight and spore production were studied.The results showed that different additives on the mycelium dry weight and spore production of T. pseudokoningii St-zn-9 were different, jujube infusion, glucose, urea, potassium dihydrogen phosphate,potato infusion and corn stalk infusionhave significant difference compared with control.Combination cultivation D（add corn stalk infusion and urea） was best,spore production mayachieved（14.17±0.38）×10^7/mL.

关 键 词：木霉 营养物质 菌丝生长 产孢量 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]