活性氧介导的植物蛋白质氧化修饰研究进展  

Research Progress on the Oxidative Modification of Plant Proteins Mediated by Reactive Oxygen Species

作　　者：周文菲 白娟[1] 龚春梅[1] ZHOU Wen-fei BAI Juan GONG Chun-mei(College of Life Sciences, Northwest A＆F University, Yangling 712100)

机构地区：[1]西北农林科技大学生命科学学院,杨凌712100

出　　处：《生物技术通报》2017年第4期8-18,共11页Biotechnology Bulletin

基　　金：国家自然科学基金面上项目(31370599)

摘　　要：蛋白质翻译后修饰是机体蛋白发挥各种功能的重要先行步骤。逆境胁迫下活性氧可对氧化还原敏感的蛋白质进行可逆和不可逆的氧化修饰。可逆氧化修饰对逆境下植物生物功能的正常发挥乃至适应都至关重要,目前对活性氧调节植物蛋白质的可逆氧化修饰研究取得了相应进展。综述了植物蛋白质氧化修饰的方式位点及参与蛋白的调节机理,旨在阐明蛋白质可逆氧化修饰对植物抵御逆境造成的氧化胁迫的重大意义,同时概括对蛋白质可逆氧化修饰研究的有效方法。当前可以通过特定位点的突变实验和蛋白质组学的方法确定有无可逆氧化修饰并测定氧化修饰程度;未来以期通过综合实验控制参与反应的蛋白质来解析其还原及再生机理。Post-translational modification of protein is an important process for precursor protein to its carrying-out function. Some redoxsensitive proteins can be modified reversibly or irreversibly by reactive oxygen species in stress conditions. At present,research on oxidativemodification of plants is still in the initial stage. With growing demand on plant developing the ability to defense the harsh environment,moreattentions have been focused on the reversible oxidative modification of proteins caused by reactive oxygen species,and some correspondingprogresses have been finished in its mechanism and research methods. This review describes the way and sites of oxidative modification of plantproteins and regulation mechanism of various oxidoreductase,aiming at illuminating the significance of reversible oxidative modification inthe oxidative stress resulted from defensing the stress to plant. The review also summarizes the effective methods for studying the reversibleoxidative modification of proteins. Currently,via mutations of specific sites and proteomics it is feasible to determine whether or not there arereversible oxidative modifications and in which extent the oxidative modification is. Moreover,in future,it is aimed to dissect the reduction andregeneration mechanism by comprehensive experiments to control the proteins involved in reactions.

关 键 词：活性氧 蛋白质 翻译后修饰 甲硫氨酸 半胱氨酸 

分 类 号：Q946[生物学—植物学]