马尾松响应松材线虫侵染的基因动态表达变化  

The Dynamic Change of Gene Expression from Pinus massoniana in Response to Bursaphelenchus xylophilus Infestation

作　　者：谢婉凤[1,2] 李慧敏[1] 黄爱珍[1] 冯丽贞[1] 张飞萍[1] 

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学金山学院,福建福州350002

出　　处：《福建农业学报》2017年第4期403-409,共7页Fujian Journal of Agricultural Sciences

基　　金：福建省财政厅资助项目(K81139238;K8911010)

摘　　要：松材线虫病是一种严重危害马尾松生长的流行性病害,可导致马尾松枯萎死亡。为揭示该过程中基因的表达变化行为,本研究利用荧光定量PCR技术检测了松材线虫侵染不同天数下的马尾松较其对照样本中病原识别、抗逆调节、次生代谢、解毒作用及生长素响应等相关基因的表达变化。结果显示,除了与病原识别相关的CC-NBS-LRR抗性蛋白基因的表达随侵染天数的增加而增强之外,其他基因则在侵染2d的马尾松样本中的表达水平最高,且明显高于未受侵染的对照马尾松样本,随后在侵染3天的马尾松中的表达又低于对照样本。此外,黄酮-3-羟化酶和细胞色素P450基因的表达随着侵染虫量的增加呈先上调后下调的变化方式。通过本研究初步揭示了马尾松响应松材线虫侵染的基因表达变化模式。Pine wilt disease(PWD)is a devastating disease,which affects on the growth of masson pine(Pinus massoniana),often leading to withering and death.To reveal the change of gene expression pattern on this process,the gene dynamic expression from the P.massonianainfestation with nematode(Bursaphelenchus xylophilus)for1,2,and 3days was studied.The genes,which were relevant to pathogen recognize,stress regulation,secondary metabolism,detoxification,and auxin-response were concerned.The results showed that most of the tested genes were presented highest expression level on the P.massoniana with infestation for two days,it was then downregulated on these groups with 3-day infestation.Besides,the gene expression of CC-NBS-LRR resistance protein was continually increased in the treated P.massoniana compared with the corresponding control group.Additionally,genes expression of flavonoid 3-hydroxylase and cytochrome P450 were firstly up-regulated and then down-regulated on the P.massoniana respectively infected by graded increasing nematode qualities.The current findings preliminarily revealed the gene expression pattern on the P.massoniana in response to B.xylophilus infestation.

关 键 词：马尾松 松材线虫 基因表达 病害响应 

分 类 号：S763.3[农业科学—森林保护学]