鱼腥草抗单纯疱疹病毒作用机制研究  

Study on antiviral effects ofHouttuyniaon herpes simplex

作　　者：周良斌[1] 

机构地区：[1]黄冈职业技术学院,湖北黄冈438002

出　　处：《中国饲料》2017年第10期10-16,共7页China Feed

摘　　要：利用体外试验研究鱼腥草是否对第二型单纯疱疹病毒(HSV-2)感染具有抑制作用,并进一步探讨其抑制单纯疱疹病毒的效用与机制。结果表明:利用溶斑减少试验发现,鱼腥草初萃取物可抑制HSV-1、HSV-2及阿昔洛韦抗药性HSV-1(HSV-AR)。将鱼腥草初萃取物依据分子量分成1000 Da以下、1000~3000 Da、3000 Da以上三部分,发现三部分皆具有抗病毒的成分,其中以1000 Da以下的部分抑制效果最好。分析六种鱼腥草成分作用发现,B、E及F具有抗HSV活性。其中B可以同时抑制病毒进入细胞以及NF-κB活化。可见,鱼腥草可直接和病毒颗粒作用而抑制病毒进入细胞。在病毒进入细胞后,鱼腥草通过抑制细胞NF-κB活化而减少NF-κB结合至HSV的ICP0基因启动子而抑制HSV复制。In vitro experimental was used to study cordate Houttuynia whether has inhibitory effect on type 2 herpes simplex virus infection, and further explore its inhibition mechanism and utility of herpes simplex virus.The results showed that using dissolve freckle reduction experiment found that its original extract can inhibit the following three kinds of viruses： less than 1000 Da,1000 ？3000 Da,more than 3000 Da.Among them, less than 1000 Da can show the best effect.Through analysis of six kinds of cordate Houttuynia, it’ s found that B,E and F had anti HSV activity,B can suppress the virus into the cell.Cordate Houttuynia can suppress the virus and virus particles directly into the cell.After the virus into cell, cordate houttuynia by inhibiting NF-κB cell to reduce NF-κB combine, then HSVICPO gene start and inhibit the replication of HSV.

关 键 词：单纯疱疹病毒 鱼腥草 抗病毒作用 

分 类 号：S816.3[农业科学—饲料科学]