沙门菌sopB基因缺失诱导小鼠巨噬细胞死亡的研究  

Study on Murine Macrophage Death Induced by Salmonella sopB Gene Delation

作　　者：戚帅[1] 秦晓霞[1] 胡桂秋[1] 张杰 于水星 陈巍[1] 韩文瑜[1] 杨勇军[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《动物医学进展》2017年第5期38-42,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助项目(3A411V246604)

摘　　要：沙门菌是一种胞内寄生菌,能够引起人和多种动物疾病,是一种世界范围内的重要疾病。为了更好理解在沙门氏菌感染过程中效应蛋白SopB对巨噬细胞存活的影响,利用鼠伤寒沙门菌SL1344和sopB基因缺失菌株分别感染小鼠骨髓来源的巨噬细胞,对2株菌在巨噬细胞内存活情况进行统计。随后用Western blot检测DNA修复酶PARP的剪切情况,通过ELISA检测培养上清中细胞因子和趋化因子的分泌情况。结果发现sopB基因缺失之后,沙门菌在巨噬细胞内的存活显著降低;培养上清中细胞因子和趋化因子分泌增强,巨噬细胞坏死增加。说明sopB基因缺失可促进巨噬细胞坏死。Salmonella is an intracellular pathogen which infection can cause a range of diseases in human and animals worldwide.To investigate the effect of SopB in Salmonella-induced cell death,in this study, bone marrow derived macrophages （BMDMs） were infected with SL1344 and AsopB respectively, the interacellular survivals of these two bacteria in BMDMs were investigated. Subsequently, the expression of cleaved PARP was detected via Western blot and the secretion of cytokine and chemokine were examined via ELISA.We found that AsopB has a significant lower intracellular survival; however,the expression of cleaved PARP and the secretion of inflammatory cytokine were increased.Collectively,these results indicated that the SopB deletion could significantly promote the necrosis of macrophage.

关 键 词：沙门菌 SopB 巨噬细胞 细胞死亡 坏死 

分 类 号：S852.4[农业科学—基础兽医学]