乏氧对IL-17调控牙周膜细胞RANKL及OPG表达的影响  

Effect of IL-17 on modulating the expression of RANKL and OPG in periodontal ligament cells under hypoxia

作　　者：茅飞飞 李璐[1,2] 王振婷[1] 王玉珍[1] 徐艳[1,2] 

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029 [2]南京医科大学附属口腔医院牙周科,江苏南京210029

出　　处：《口腔医学》2017年第5期385-389,共5页Stomatology

基　　金：国家自然科学基金(81470749);江苏省高等学校大学生创新创业训练计划资助项目(201410312055X);江苏省高校自然科学研究面上项目(14KJD320001)

摘　　要：目的探究不同乏氧条件下IL-17对人牙周膜成纤维细胞RANKL及OPG表达的影响。方法在常氧(氧体积分数20%)及不同乏氧条件下:轻度乏氧(氧体积分数10%)、中度乏氧(氧体积分数5%)、重度乏氧(氧体积分数2%)IL-17处理人牙周膜成纤维细胞24 h。通过CCK8比色法检测细胞增殖情况,实时定量RT-PCR和Western-blot方法检测细胞中骨吸收相关因子RANKL及骨保护因子OPG的表达水平。结果氧体积分数10%、5%时,人牙周膜成纤维细胞的增殖与时间呈正相关关系,氧体积分数2%时,细胞增殖受到明显抑制。乏氧培养后随氧体积分数的降低,人牙周膜成纤维细胞RANKL蛋白及mRNA的表达升高,OPG的蛋白及mRNA表达呈现先升后降的趋势。结论当氧体积分数在5%左右时最大程度上调RANKL/OPG比例,提示中度乏氧可促进IL-17调控人牙周膜成纤维细胞骨吸收相关因子的表达,并参与牙槽骨的改建。Objective To investigate the effect of IL-17 on modulating the expression of RANKL and OPG in human periodontal liga- ment cells（hPDLCs） under hypoxia. Methods Human PDLCs were incubated in the normoxia （normal oxygen tension） and different hypoxic atmospheres （oxygen volume fraction 10%, 5%, 2%） for 24 hours. After that, cell proliferation assay was determined using CCK-8 technique. The expression levels of RANKL and OPG were determined by western blotting and real-time PCR. Results Human PDLCs proliferation increased in the normoxia and the hypoxic atmosphere of 10% 02 and 5% 02 and had a positive correlation with time in a time-dependent manner while the hPDLCs proliferation was inhibited in the hypoxic atmosphere of 2% 02. With the decrease of oxygen concentration under hypoxia, OPG mRNA and protein levels were up-regulated at first and then down-regulated meanwhile RANKL mRNA and protein levels were up-regulated after stimulated by hypoxia. Conclusions The relative RANKL/OPG expression ratios increase mostly in a hypoxic atmosphere of 5% O2, suggesting that moderate hypoxia can modulate the alveolar renlodeling via regulating the expressions of pro-osteoplastic molecules of hPDLCs through IL-17.

关 键 词：乏氧 牙周膜成纤维细胞 白介素17 低氧诱导因子 骨吸收相关因子 骨保护因子 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]