利用大肠杆菌全细胞催化赖氨酸发酵液生产1,5-戊二胺  

1,5-pentanediamine production by using Escherichia coli whole-cell biocatalysis lysine fermentation liquid

作　　者：齐雁斌 马伟超[1] 陈可泉[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《化工进展》2017年第5期1843-1847,共5页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(21390200;31440024);国家重点基础研究发展计划(2011CBA00807);国家高技术研究发展计划(2014AA021703)项目

摘　　要：1,5-戊二胺是一种具有生物活性的生物胺。赖氨酸脱羧酶可以催化L-赖氨酸生产1,5-戊二胺。为了减少生产成本,本文利用大肠杆菌AST1以赖氨酸发酵液作为底物进行全细胞催化生产1,5-戊二胺。研究转化p H、菌体浓度、转化温度、磷酸吡哆醛(PLP)添加量以及不同酸种类对转化的影响,并对菌体的重复利用性进行了研究。在最优条件下:p H6.8、转化温度37℃、PLP添加量0.1mmol/L、菌体浓度(DCW)2.5g/L,用乙酸来调节转化过程p H,可以转化含有赖氨酸123.8g/L的发酵液,得到含有86.18g/L戊二胺的转化液,转化率可达到99.61%。并且菌体在赖氨酸发酵液中重复利用5次的情况下转化率可以达到50%以上,重复利用性明显比在赖氨酸溶液中转化时强,这极大程度地节约了生产成本,为1,5-戊二胺连续工业化生产打下了基础。1,5-pentanediamine is a bioactive biogenic amine. L-lysine decarboxylase can catalyze with L-lysine to produce 1,5-pentanediamine. To reduce the production cost,whole cell catalytic production of 1,5-pentanediamine was outperformed using Escherichia coli AST1 and with lysine fermentation broth as the substrate. The effects of transformation pH, cell concentration, transformation temperature,PLP addition amount,and different kinds of acid on the transformation and the reusability of the cells were investigated. At the optimal condition,0.1mmol/L PLP,2.5g/L DCW and pH as 6.8,37℃,86.18g/L of 1,5-pentanediamine was obtained by transforming the fermentation broth containing 123.8 g/L L-lysine,and adjusting the pH using the acetic acid during conversion process. Furthermore,the cells can be reused five times and the substrate conversion rate maintained above 50% in the lysine fermentation broth. The reusability was better than that in the lysine solution,which greatly reduces the production cost and lays a foundation for 1,5-pentanediamine commercial production.

关 键 词：1 5-戊二胺 赖氨酸发酵液 全细胞转化 工业化生产 

分 类 号：TQ033[化学工程]