急性坏死性胰腺炎大鼠水通道蛋白4与脑损伤关系探讨  

Study on relationship between aquaporin-4 and cerebral injury in rats with acute necrotizing pancreatitis

作　　者：孔雷[1] 包皙婷[1] 胡皆乐[1] 李军[1] 李佑[1] 吴庆华[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院北院普外科,上海201821

出　　处：《外科理论与实践》2017年第2期139-142,共4页Journal of Surgery Concepts & Practice

基　　金：上海交通大学医学院附属瑞金医院北院院级课题基金(2014ZY11)

摘　　要：目的 :探讨水通道蛋白4(AQP4)在急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠脑组织中的变化趋势,观察其与血脑屏障通透性和脑水肿的相关性。方法:84只成年雄性SD大鼠,随机分为ANP组(n=72)和假手术组(n=12)。制备ANP大鼠模型。假手术组于造模后24 h,ANP组于注射牛磺胆酸钠造模后3、6、9、12、18和24h 6个时间点为6个组,检测脑组织含水量、血脑屏障通透性变化,Western印迹法和半定量逆转录PCR检测脑组织AQP4蛋白及m RNA表达。结果 :脑组织AQP4蛋白及m RNA在损伤后6 h开始表达上调,9、12、18和24 h依次升高(P<0.05)。AQP4蛋白及m RNA的表达与脑组织含水量均呈正相关(P<0.05)。结论:ANP大鼠的血脑屏障功能降低及脑组织AQP4蛋白及m RNA表达水平增加,可能与脑水肿的形成和发展密切相关。Objeetive To explore the change in aquaporin-4 （AQP4） in the cerebral tissue of the rats with acute necrotizing pancreatitis （ANP） and analyze the association of AQP4 with blood-brain barrier permeability and brain edema. Methods Eighty-four adult male SD rats were divided into the ANP groups （n=72） and sham operation group （n=12）. The rats with ANP were designated 3, 6, 9, 12, 18 and 24 h after pancreatitis model induced by injecting sodium tauroeholate. The brain water content in rats and the blood-brain barrier permeability at each time point were measured respectively. At the same time, the expression of AQP4 both protein and mRNA in brain tissue was detected with Western blot and semi- quantitative reverse transcription polymerase chain reaction. Results The expression of AQP4 protein and mRNA in the brain tissue was significantly up-regulated at 6 h and increased at 9, 12, 18 and 24 h successively （P〈0.05）. It was shown that the expression of AQP4 protein and mRNA was positively correlated with the brain water content （P〈0.05）. Conclusions There would be decrease in blood-brain barrier function and increase in the expression of protein and mRNA of AQP4 in the brain tissue of rats with ANP, which may be closely related to the formation and development of cerebral edema.

关 键 词：急性坏死性胰腺炎 脑损伤 水通道蛋白4 血脑屏障 

分 类 号：R657.51[医药卫生—外科学]