一只半野生病死川金丝猴肠道微生物多样性分析  

Gut Microbiota of a Dead Semi-Wild Golden Snub-Nosed Monkey in a Shennongjia Nature Reserve

作　　者：刘燕[1,2] 郑常明[1,2] 李林海[3] 张成林[1,2] 刘学锋[1,2] 姚辉 余辉亮 李莹[1,2] 杨明海[1,2] 贾婷[1,2] 卢岩[1,2] 夏茂华[1,2] 

机构地区：[1]北京动物园,北京100044 [2]圈养野生动物技术北京市重点实验室,北京100044 [3]国家林业局林产工业规划设计院,北京100010 [4]湖北省金丝猴保护研究中心,神农架442421

出　　处：《野生动物学报》2017年第2期194-199,共6页CHINESE JOURNAL OF WILDLIFE

基　　金：北京市公园管理中心科研项目:高通量测序技术分析北京动物园大熊猫肠道菌群多样性;项目编号:zx2015013;国家科技支撑计划课题(2013BAD03B01)

摘　　要：对神农架保护区大龙潭保护站1只半野生病死川金丝猴的不同部位的肠道内容物进行了微生物多样性研究。在无菌条件下取十二指肠段、小肠段、盲肠段及大肠段4个部位的肠内容物,提取微生物基因组DNA,应用高通量测序技术,对细菌和真菌多样性进行了详细的分析,比较了不同部位肠道的细菌和真菌的丰度、分布、多样性及变化趋势等。发现该川金丝猴肠道细菌主要分属于厚壁菌门(Firmicutes)、疣微菌门(Verrucomicrobia)、变形菌门(Proteobacteria)、梭杆菌门(Fusobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)和Synergistetes 7个门,真菌主要分属于子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、接合菌门(Zygomycota)以及未分类真菌(Fungi_unclassified)。细菌丰度最高的是盲肠段,细菌多样性最高的是大肠段,真菌丰度最高的是小肠段,真菌多样性最高的是大肠段。We analyzed the gut microbiota from different parts of the intestine of a dead semi-wild golden snub - nosed monkey in Dalongtan Station of Shennongjia National Nature Reserve. Intes- tinal content was sampled from the duodenum, small intestine（jejunum and ileum）, cecum, and large intestine（colon and rectum）under aseptic condition. Microbial genome DNA were extracted and sequenced by high -output sequencing. Then we analyzed the intestinal bacterial and fungal diversity and compared bacterial and fungal abundance, distribution, diversity and change trends between different parts of the intestinal. The intestinal bacteria of the golden snub - nosed monkey mainly belong to Firmicutes, Verrucomicrobia, Proteobacteria, Fusobacteria, Actinobacteria, Bacteroidetes, and Synergistetes. The intestinal fungi mainly belong to Ascomycota, Basidiomy- cota, Zygomycota, and unclassified fungi. Bacteria abundance was highest in the cecum while bacterial diversity was highest in the large intestine. Fungi were most abundant in the small intes- tine while fungus diversity was highest in the large intestine.

关 键 词：川金丝猴 肠道菌群 多样性分析 投食群 神农架 

分 类 号：S852.61[农业科学—基础兽医学]