不同生理状态下银荷方药物制备物黄酮类成分的质量表征特性研究  

Quality representation characteristics of flavonoids in pharmaceutical preparation of Yinhe Fang under different physiological conditions

作　　者：张芳[1] 权建野 陈梦杰 刘璐[1] 李倩[2] 吕珊[1] 张元元[1] 陈唯[1] 杨元[1] 石任兵[1] 

机构地区：[1]北京中医药大学中药学院国家中医药管理局中药经典名方有效物质发现重点实验室,北京100102 [2]首都医科大学中医药学院

出　　处：《北京中医药大学学报》2017年第4期322-327,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：十二五国家科技支撑计划项目(No.2012BAI29B06)

摘　　要：目的建立银荷方药物制备物及大鼠血浆中槲皮素、山奈酚、异鼠李素的含量测定方法,以研究银荷方药物制备物黄酮类成分的质量表征特性。方法制备物和血浆样品均加酸进行水解,并用乙醚-丙酮(14∶1)萃取法处理血浆样品,采用HPLC分析,以C_(18)为固定相,流动相为乙腈-0.2%磷酸水溶液梯度洗脱,在270 nm检测黄酮类苷元槲皮素、山奈酚、异鼠李素的含量。结果经方法学考证,槲皮素、山奈酚、异鼠李素的线性范围分别为1.49~29.70 mg/L(r=0.999 3),0.65~12.90mg/L(r=0.999 5),0.47~9.40 mg/L(r=0.999 0),日内、日间精密度RSD≤5.85%,血浆样品冻融稳定性良好,室温放置10 h,在-80℃条件下保存1个月的稳定性均良好,符合生物样品分析要求。槲皮素、山奈酚、异鼠李素在正常组中的血药浓度分别为7.25、1.71、3.73 mg/L,在血栓组中血药浓度分别为8.53、2.64、5.69 mg/L,在抗凝组中血药浓度分别为8.32、2.76、4.56 mg/L。结论本文所建立的检测方法适用于银荷方药物制备物黄酮类成分槲皮素、山奈酚和异鼠李素的体内含量测定,并可应用于分析黄酮类成分质量表征特性,为基于黄酮类成分的银荷方药物质量精准控制提供科学依据。Objective To investigate the quality representation characteristics of flavonoids in pharmaceutical preparations of Yinhe Fang through developing Yinhe Fang preparation and determining quercetin,kaempferol and isorhamnetin in rat ＇s blood plasma. Methods Preparation and plasma samples were hydrolyzed with acid,and plasma samples were treated with ether acetone（ 14 ∶ 1）extraction. HPLC was used to analyze the concentration of flavonoid aglycones quercetin,kaempferol and isorhamnetin. C18 was used as stationary phase and acetonitrile-0. 2% phosphoric acid solution as mobile phase in gradient elution at 270 nm detection wavelength. Results The linear concentrationranges of quercetin,kaempferol and isorhamnetin were 1. 49 - 29. 70 mg/L（ r = 0. 999 3）,0. 65 -12. 90 mg/L（ r = 0. 999 5）,and 0. 47 - 9. 40 mg/L（ r = 0. 999 0） respectively. RSD values were all less than 5. 85%. Plasma samples showed good stability after placed at room temperature for 10 h and then frozen at-80℃ for one month,which were consistent with the requirement of biological sample analysis. The serum concentration of quercetin,kaempferol and isorhamnetin were 7. 25,1. 71,and3. 73 mg/L respectively in the normal group; 8. 53,2. 64,and 5. 69 mg/L in the thrombus model group;8. 32,2. 76,and 4. 56 mg/L in the anticoagulant model group. Conclusion This method was developed for detecting flavonoids quercetin,kaempferol and isorhamnetin in pharmaceutical preparations of Yinhe Fang in vivo,and can be used to analyze the quality representation characteristics of flavonoids.It provides a scientific evidence for accurate quality control of Yinhe Fang based on flavonoids.

关 键 词：银荷方药物制备物 槲皮素 山奈酚 异鼠李素 血栓 抗凝 

分 类 号：R284.1[医药卫生—中药学]