普瑞巴林抑制神经病理性疼痛大鼠脊髓背角神经细胞c-fos的表达  

Pregabalin reduce the expression of c-fos in spinal dorsal horn of the rat with neuropathic pain

作　　者：金梅花[1] 马丽[2] 苗丽竹 张平[3] 

机构地区：[1]大连市妇幼保健院暨大连医科大学附属大连市妇产医院麻醉科,116000 [2]大连市第二人民医院麻醉科 [3]延边大学附属医院疼痛科,133000

出　　处：《中国医师进修杂志》2017年第4期363-366,共4页Chinese Journal of Postgraduates of Medicine

摘　　要：目的 观察普瑞巴林对神经病理性疼痛大鼠脊髓背角神经细胞c-fos蛋白表达的影响.方法 健康雄性Wister大鼠30只,采用随机数字表法分为假手术组、模型对照组和普瑞巴林组,每组10只.观察普瑞巴林对神经病理性疼痛大鼠热痛阈和脊髓灰质背角神经元中c-fos蛋白表达的影响.结果 模型对照组术后第3、4、5、6、7、10、14天热痛阈值均低于同时点假手术组和普瑞巴林组,差异有统计学意义（P＜0.05）.普瑞巴林组术后第2天起热痛阈值低于假手术组,但差异无统计学意义（P＞0.05）.术后第14天免疫组化结果表明,假手术组、模型对照组、普瑞巴林组Fos样免疫反应阳性神经元细胞数目分别为（16.4±0.6）、（66.7±3.3）、（22.8±1.5）个,模型对照组阳性细胞数明显高于普瑞巴林组和假手术组（P＜ 0.05）.结论 普瑞巴林对神经病理性疼痛的大鼠具有镇痛作用,并且活化外周神经损伤和感染引起的脊髓胶质细胞,减少脊髓背角神经元对痛觉传入的伤害性刺激.Objective To explore the role of pregabalin on the expression of c-fos in spinal dorsal horn of the rat with neuropathic pain.Methods Thirty Wister rats （male） were divided into shamoperated group,model group and pregabalin group,with 10 cases in each group.The effect of the pregabalin on the heat pain threshold and expression of c-fos in spinal dorsal horn of the rat with neuropathic pain were observed.Results The heat pain threshold in model group at 3rd,4th,5th,6th,7th,10th and 14th day after operation was significantly lower than that in sham-operated group and pregabalin group at same time （P ＜ 0.05）.The heat pain threshold in pregabalin group was lower than that in sham-operated group from 2nd day after operation,but there was no significant difference （P ＞0.05）.At the 14th day after operation,the number of Fos-like-immunoreactivity （FLI） positive cells in sham-operated group,model group and pregabalin group was （16.4 ± 0.6）,（66.7 ± 3.3） and （22.8 ± 1.5）cases,and the number of FLI positive cells in model group was significantly higher than that in shamoperated group and pregabalin group （P ＜ 0.05）.Conclusions Pregabalin has analgesic activity on rat with neuropathic pain.Pregabalin activates spinal cord glial cells raisedby the injured peripheral nerve and infection,and also reduces the noxious stimulation of afferent pain to spinal dorsal horn neurons.

关 键 词：神经痛 原癌基因蛋白质C-FOS 普瑞巴林 

分 类 号：R965[医药卫生—药理学]