HIV-1感染者慢性期治疗前后CD8＋ Tsem的特点研究  

Characteristics of CD8^＋ stem memory T cells in patients with chronic HIV-1 infection during antiret- roviral therapy

作　　者：陆小凡[1] 夏欢[1] 粟斌[1] 吴昊[1] 张彤[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染中心、艾滋病研究北京市重点实验室,100069

出　　处：《中华微生物学和免疫学杂志》2017年第4期250-254,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81501732）;艾滋病研究北京市重点实验室（BZ0089）

摘　　要：目的探讨HIV-1感染者慢性期治疗前后CD8^＋Tscm（stem memory Tcell）的特点及其与疾病进展的相关性。方法选取36例HIV-1慢性期感染者和20名健康对照者。用流式细胞术检测健康对照者和HIV-1感染者抗病毒治疗前后CD8^＋Tscm的比例和数量。分析CD8^＋Tscm与其他CD8^＋T细胞亚群和疾病进展指标（CIM^＋T细胞数量、病毒载量和T细胞活化水平）的相关性。结果抗病毒治疗前后，HIV-1慢性期感染者CD8^＋Tscm的比例和数量均无显著性变化。HIV-1慢性期感染者CD8^＋Tscm的比例和数量均与CIM^＋Tscm的比例和数量呈正相关性。CD8^＋Tscm的比例与CD8^＋Tcm（central memory T cell）的比例成正比．与CD8^＋Tern（effector memory T cell）的比例成反比。此外，在抗病毒治疗前，HIV-1慢性期感染者CD8^＋Tscm的比例与病毒载量呈负相关。结论CD8^＋Tscm参与维持其他CD8^＋T细胞亚群的稳态：CD8^＋Tscm参与抑制病毒的复制。Objective To investigate the characteristics of CD8^＋ stem memory T cells （CD8＋ Tscm） in patients with chronic HIV-1 infection before and ＇after antiretroviral therapy （ART） and to analyze their associations with progression of HIV-1 infection. Methods Thirty-six patients with chronic HIV-1 in- fection and 20 healthy subjects were enrolled in this study. Flow cytometry was peffomled to detect the per- centages and absolute numbers of CD8＋Tscm in patients with chronic HIV-1 infection before and after antiret- roviral therapy （ART） as well as in healthy subjects. Correlation analysis was used to demonstrate the rela- tionships between CD8^＋Tscm and markers for progression of HIV-1 infection （ CD4^＋T cell count, HIV-1 viral load and level of activated T cells）. Results The percentages and the absolute numbers of CD8^＋Tscm in patients with chronic HIV-1 infection had no significant change before and after ART. They were respectively positively correlated with the percentages and the absolute numbers of CD4^＋Tscm. The percentage of CD8^＋ Tscm was proportional to the percentage of CD8^＋ central memory T cells （ CD8^＋Tcm ） , but was inversely pro- portional to the percentage of CD8^＋ effector memory T ceils （CD8^＋ Tern）. In addition, the percentages of CD8＋Tscm in patients with HIV-1 infection were negatively correlated with the viral loads before ART. Con- clusion CD8^＋Tscm are responsible for maintaining the homeostasis of other CD8^＋T cell subsets. CD8^＋Tscm play an important role in inhibiting viral replication.

关 键 词：H1V-1慢性期感染 抗病毒治疗 CD8^+ Tscm 

分 类 号：R512.91[医药卫生—内科学]