高生物量富硒产朊假丝酵母菌株的选育  

Screening of high-biomass and selenium-enriched Candida utilis

作　　者：邹艳[1] 李俊杰[1] 夏诗棋 管斌[1] 孔青[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国酿造》2017年第5期85-89,共5页China Brewing

基　　金："十二五"农村领域国家科技计划(2013BAD10B02-06);山东省科技计划项目(2014GSF121029)

摘　　要：从5株优良的发酵酵母菌中筛选出耐硒和富硒能力较好的产朊假丝酵母,采用耐酸驯化和梯度浓度的耐硒驯化增加菌株的抗性。研究表明,产朊假丝酵母Ⅰ的富硒能力更好。在此基础上,经复合诱变、亚硒酸钠抗性平板初筛和摇瓶复筛,筛选出生物量和含硒量都较高的菌株D-5,再将突变株D-5进行紫外诱变,筛选出1株高生物量和高含硒量的菌株U-16,其菌株生物量为5.86 g/L,总硒量为1 575.40μg/g,有机硒量为1 500.90μg/g,其有机硒占胞内总硒比重的95.26%。与出发菌株相比,筛选菌株的生物量提高了67.90%,有机硒量增加了95.95%。Candida utilis, with better selenium-endurance and selenium-enriched ability was screened from 5 excellent yeast strains, after acid acclimation and selenium concentration gradient domestication. Results showed that C. utilis I showed better selenium-enriched ability. On that basis, the strain was treated by compound mutation, sodium selenite resistance plate primarily screening and shake-flask secondary screening, a selenium-en- riched strain D-5 with higher biomass was isolated. The strain D-5 was treated with UV mutagenesis, a selenium-enriched and high biomass strain U-16 was selected by using the same method. The biomass of strain U-16 was 5.86 g/L, and the total selenium and organic selenium content was 1 575.40μg/g and 1 500.90μg/g, respectively. The organic selenium content accounted for 95.26% of the total selenium in the cell. Compared with the original strain, the biomass and organic selenium was 67.90% higher and 95.95% higher, respectively.

关 键 词：有机硒 产朊假丝酵母 诱变 生物量 

分 类 号：Q93-331[生物学—微生物学]