不同归经补益中药对骨转移癌CXCR4表达的影响  

Effects of Different Channel Tropism Tonic Herbs on CXCR4 Expression in Bone Metastasis Cancer

作　　者：林荔钦[1] 朱世杰[1] 

机构地区：[1]中国中医科学院望京医院肿瘤科,北京100102

出　　处：《中国中西医结合杂志》2017年第5期552-556,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173374)

摘　　要：目的观察不同归经补益中药对骨转移癌骨趋化因子受体4(CXCR4)的抑制及机制。方法采用转移性大鼠乳腺癌细胞,按Medhurst方法复制骨转移癌模型。40只大鼠按照体重分层,采用随机数字表法分为假手术组、模型组、肾经组、肺经组和肝经组,每组8只。造模后肾经组(补骨脂、肉苁蓉及益智仁)、肝经组(熊胆粉)、肺经组(党参、黄芪及百合)给药量为10 mL/(kg·d),相当于生药45 g/(kg·d)灌胃,假手术组及模型组给予去离子水灌胃,持续20天。观察不同归经补益中药的作用,通过影像学方法进行评分、测量肿瘤体积,HE染色观察形态,免疫组化法检测CXCR4表达并进行半定量分析。结果骨转移癌模型出现骨质破坏和肿瘤生长等表现。与假手术组比较,模型组肿瘤体积增大(P<0.01),模型组骨质破坏严重,评分升高(P<0.01),CXCR4表达升高(P<0.01)。与模型组比较,肾经组肿瘤体积缩小(P<0.05),骨质破坏明显减轻,评分降低(P<0.05),CXCR4表达明显下降(P<0.01);肺经组和肝经组上述指标与模型组比较,差异均无统计学意义(P>0.05)。结论归肾经中药对骨转移癌灶的生长具有一定的抑制作用,其机制可能通过阻断SDF-1/CXCR4生物学轴及下游信号通路,达到抗肿瘤定向侵袭和增殖的目的。归肺经、归肝经药物不存在类似作用,不同归经中药对机体的作用存在靶向特异性。Objective To observe inhibition and mechanisms of different channel tropism tonic herbs on CXCR4 expression. Methods Bone metastatic carcinoma model was established according to Medhurst method with metastatic breast cancer cells in rats. Forty rats were stratified by body weight. Then, the rats were divided into 5 groups according to random digit table, i.e., the sham-operated group, the model group, Shen channel group （ Fructus Psoraleae, Cistanche deserticola, Fructus Alpiniae oxyphyllae）, Fei channel group （ Codonopsis pilosula , Astragalus mongholicus , Lilium brownfi ） , and Gan channel group （Bear Bile Powder）, 8 in each group. After modeling rats in Shen channel group, Fei chan- nel group, and Gan channel group were administered with corresponding decoctions （10 mL · kg -1 · d -1） by gastrogavage （equivalent to 10 times that 45 g · kg-1 · d-1 crude drugs）. Equal volume of deionized water was administered to rats in the sham-operated group and the model group by gastrogavage. All medications lasted for 20 days. Effects of different channel tropism tonic herbs were observed. Imaging methods were applied to score and measure the tumor volume, and morphological changes were ob- served by HE staining. In addition, expressions of CXC chemokine receptors （CXCR4） were semi-quanti- tatively detected using immunohistochemistry. Results In bone metastatic carcinoma model bone de- struction and tumor growth occurred. Compared with the sham-operated group, tumor volume were in- creased significantly （P 〈0.01 ）, bone destruction were severer and scores increased （P 〈0.01 ）, as well as CXCR4 expression levels were significantly higher in the model group （P 〈0.01 ）. Compared with the model group, bone destruction was obviously attenuated, and scores decreased （P 〈0.05）, tumor volume was reduced （P 〈0.05）, and CXCR4 expression level was obviously lowered （P 〈0.01 ） in Shen channel group. However, there was no significant difference in aforesaid parameters bet

关 键 词：骨趋化因子受体4 补益中药 归经 

分 类 号：R273[医药卫生—中西医结合]