早期应用疏风清热合剂调节急性相蛋白和VEGF促进骨折愈合的实验研究  

Early Application of Shufeng Qingre Mixture for Promoting Fracture Healing from Expressions of Acute Phase Proteins and VEGF

作　　者：袁晓芳[1,2] 张建芳[1] 万全庆[3] 

机构地区：[1]浙江中医药大学第三临床医学院,杭州310053 [2]江苏省常州市第一人民医院康复科,江苏213002 [3]浙江中医药大学附属第三医院椎间盘中心,杭州310005

出　　处：《中国中西医结合杂志》2017年第5期584-588,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省自然科学基金项目(No.LY14H270012);姚新苗全国名老中医药专家传承工作室建设项目(国中医药人教发2014〗20号)

摘　　要：目的研究不同时间段骨折处X线摄片评分和C反应蛋白(CRP)、纤维蛋白原(FIB)及血管内皮细胞生长因子(VEGF)在血液中的表达情况,探讨早期应用疏风清热合剂促进骨折愈合的机制。方法建立家兔双侧桡骨骨折模型,将造模成功后的33只家兔按随机数字表法分为模型对照组、生理盐水组和疏风清热合剂组,每组11只,另设空白对照组11只。疏风清热合剂组和生理盐水组于造模后10天内分别给予疏风清热合剂[10 mL/(kg·d)]和等体积的生理盐水灌胃。造模后第28、42天检测双侧桡骨进行X线摄片,第1、3、7、14天检测血液中CRP、FIB表达,第7、14、28天检测血液中VEGF表达情况。结果(1)与模型对照组比较,疏风清热合剂组第28、42天骨折部位X线评分较高(P<0.01),且高于生理盐水组(P<0.05)。(2)与本组造模后第3天比较,模型对照组、生理盐水和疏风清热合剂组第7天CRP、FIB均表达较低(P<0.05)。与空白对照组比较,模型对照组第1、3、7天血浆CRP、FIB表达水平均升高(P<0.01);与模型对照组比较,疏风清热合剂组第3、7天时间点CRP、FIB表达降低(P<0.05,P<0.01);与生理盐水组比较,疏风清热合剂组在第3、7天时间点CRP、FIB表达降低(P<0.01)。(3)与本组造模后第14天比较,模型对照组、生理盐水和疏风清热合剂组第7、28天VEGF表达降低(P<0.05,P<0.01)。与空白对照组比较,模型对照组第7、14、28天VEGF表达均升高(P<0.01);与模型对照组比较,疏风清热合剂组第7、14、28天VEGF表达较高(P<0.01),且高于生理盐水组(P<0.01)。结论早期应用疏风清热合剂可以促进新西兰家兔桡骨骨折的愈合,其作用机制可能是通过降低急性相蛋白CRP、FIB表达以及促进血液中VEGF的高表达实现。Objective To observe the mechanism of Shufeng Qingre Mixture （SQM） for promo- ting fracture healing by studying X-ray score at different time points and the expressions of C reactive protein （CRP）, fibrinogen （FIB）, vascular endothelial growth factor （VEGF） in blood. Methods Bilateral radial fracture model was set up. Totally successfully modeled 33 rabbits were divided into the model con- trol group, the normal saline （NS） group, and the SQM group according to random number table, 11 in each group. Besides, a blank control group was set up consisting of 11 rabbits. SQM （10 mL · kg-1 · d-1） was administered to rabbits in the SQM group. Equal volume of NS was administered to rabbits in the NS group. X-ray of bilateral radius was performed at day 28 and 42 after modeling. The expression levels of CRP and FIB in blood were tested by ELISA at day 1,3,7, and 14. The expression level of VEGF was detected at day 7, 14, and 28. Results （1） Compared with the model control group, the X-ray scores were higher in the SQM group at day 28 and 42 （P 〈0.01）. It was higher in the SQM group than in the NS group （P 〈0.05）. （2） Compared with 3-day intervention in the same group, the expression levels of CRP and FIB were lower in the model control group, the NS group, and the SQM group （P 〈0.05）. Compared with the blank control group, the expression levels of CRP and FIB were all elevated in model control group at day 1,3, and 7 （P 〈0.01 ）. Compared with model control group, the expression levels of CRP and FIB were significantly reduced in the SQM group at day 3 and 7 （P 〈0.05, P 〈0.01）. Compared with the NS group, the expression levels of CRP and FIB decreased in the SQM group at day 3 and 7 （P 〈 0.01 ）. （3） Compared with 14-day intervention in the same group, the expression level of VEGF was low- er in the 3 groups at day 7 and 28 （P〈0.05, P〈0.01）. Compared with the blank control group, the ex- pression level of VEGF increased in the model control

关 键 词：骨折愈合 疏风清热合剂 C反应蛋白 纤维蛋白原 血管内皮细胞生长因子 

分 类 号：R285.5[医药卫生—中药学]