海南省12株脑膜炎奈瑟菌分型与耐药性分析  

Typing and antibiotic resistance analysis of 12 Neisseria meningitidisisolates in Hainan province

作　　者：王少玲[1] 李丹丹[1] 黄雅靖[1] 叶浩然[1] 吴捷[1] 莫少雄[1] 曾祥洁[1] 李沅[1] 吴维学[1] 

机构地区：[1]海南省疾病预防控制中心检验检测所微生物检验科,海南海口570203

出　　处：《现代预防医学》2017年第10期1855-1858,1866,共5页Modern Preventive Medicine

基　　金：海南省自然科学基金(No.814339);海南省卫计委资助项目(No.琼卫2013资助-057号);国家科技重大专项课题(2013ZX10004-203-002-006)

摘　　要：目的了解海南省脑膜炎奈瑟菌菌型和对抗生素的敏感性,为临床治疗和流脑防治提供科学依据。方法用玻片凝集法和聚合酶链反应(PCR)对本实验室2010-2015年收集的分离自病人和健康带菌者菌株进行血清分型和基因分群;用药敏纸片法和E-test试纸条法进行14种抗生素敏感性试验。结果共有12株脑膜炎奈瑟菌,其中10株血清分型结果与基因分群结果一致,2株血清不可分群菌株经PCR检测为C群和B群。3株患者分离株中,C群2株,B群1株。9株健康者分离株基因分群为B群3株,W135群、Y群各2株,C群和不可分群各1株。所有菌株对氨苄西林、头孢噻肟、头孢曲松、美罗培南、氯霉素、利福平、阿奇霉素、米诺环素均敏感。1株C群患者菌株对青霉素敏感性降低。83.3%(10/12)菌株对磺胺和复方新诺明均耐药。对环丙沙星、萘啶酸、左氧氟沙星的耐药率分别为66.7%(8/12)、58.3%(7/12)、25%(3/12)。结论海南省2010-2015年流脑的流行菌群是C群和B群,健康带菌者以B群、W135群和Y群为主。大部分菌株对复方新诺明、环丙沙星等磺胺类和喹诺酮类药物耐药。Objective To study the types and antibiotic resistance of Neisseria meningitides （Nm） in Hainan province, and to provide reference for clinical treatment and the prevention and control of Epidemic eerebrospinal meningitis （ECM）. Methods The serotypes and genotypes of Nmeollected and isolated from patients and healthy carriers during 2010-2015 in our laboratory were checked by slide agglutination test and palymerase chain reaction （PCR）. The susceptibility of these strains to 14 antibiotics were tested by E-test and Kirby-Bauer method according to the Clinical and Laboratory Standard Institute guidelines. ResMts 12 strains of Nmwere identified. Among them,10 had consistent serotyping and genotyping results, and 2 wereundeteeted with serotyping but identified by PCR as group C and group B respectively. Among the 3 strains from patients,one was of B serogroup and 2 of C serogroups. Among the 9 strains from healthy carriers, 3 was of B genogroup, 2 of W135, 2 of Y, 1 of C and 1 was not genotyped. All the Nm strains were sensitive to ampieillin, eefotaxime, eeftriaxone, meropenem, chloramphenieol, rifampiein, azithromycin and minocyeline. One strain of serogroup C isolated from patient was not sensitive to penicillin. 83.3%（10/12） of strains were resistant to sulfamethoxazole and eotrimoxazole. The resistant rates to eiprofloxaein, nalidixie acid and levofloxacin were 66.7% （8/12）, 58.3%（7/12） and 33.3% （4/12）, respectively. Conclusion Serogranp C and serogroup B were the epidemic strains of ECM in Hainan during 2010-2015. Seroganps B,Y and W135 dominated in the healthy earners. Most strains wereresistant to sulfonamides and fluoroquinones such as eotrimoxazole and eiprofloxaein.

关 键 词：脑膜炎奈瑟菌 血清型 基因群 抗生素 耐药性 

分 类 号：R378[医药卫生—病原生物学]