“黑质/苍白球-丘脑-皮质”通路5-羟色胺_(1B)受体及多巴胺1型受体在大鼠运动疲劳调控中的作用  

The modulating effect of 5-HT_(1B) and D_1DR receptors in neurons within “SNr/GPi-VL-SMA” pathway on exercise induced fatigue

作　　者：胡琰茹[1,2] 刘晓莉[1] 乔德才[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875 [2]中南大学体育教研部

出　　处：《中国康复医学杂志》2017年第5期501-508,共8页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(31171138);湖南省体育科学学会重点资助项目(2015HN016)

摘　　要：目的:通过观察运动大鼠力竭前后"黑质/苍白球-丘脑-皮质"通路各核团5-羟色胺1B受体(5-HT_(1B))及多巴胺1型受体(D_1DR)的蛋白表达水平,探讨"黑质/苍白球-丘脑-皮质"通路调控运动疲劳发生、发展的中枢机制。方法:24只雄性Wistar大鼠,随机分为安静对照组、力竭即刻组、恢复90min组,每组8只。采用免疫组织化学技术对不同组别5-HT_(1B)和D_1DR的蛋白表达水平进行检测。结果:与安静组对照组相比,力竭即刻组、恢复90min组大鼠黑质网状部及苍白球内侧部5-HT_(1B)受体阳性细胞R值及平均光密度(AOD)值均显著减少(P<0.05),D_1DR受体阳性细胞R值及AOD值均显著增加(P<0.05);力竭即刻组、恢复90min组大鼠丘脑腹外侧核5-HT_(1B)受体阳性细胞R值及AOD值均显著增加(P<0.05,P<0.01);在大鼠皮质辅助运动区,力竭即刻组、恢复90min组5-HT_(1B)受体阳性细胞R值及AOD值均显著增加(P<0.05),D_1DR受体阳性细胞R值及AOD值均显著减少(P<0.05)。结论:力竭运动过程中"黑质网状部/苍白球内侧部-丘脑-皮质"各核团5-HT_(1B)及D_1DR受体蛋白表达变化引起神经递质释放量改变,进而影响神经元电活动兴奋性,可能是"黑质网状部/苍白球内侧部-丘脑-皮质"通路调节运动疲劳及运动能力的重要途径之一。Objective： To observe the changes of 5-HT1B and D1DR expression in nucleus of “SNr/GPi-VL-SMA” pathway in pre and post-exhaustive exercise, and investigate the central mechanism of “SNr/GPi-VL-SMA” pathway modulating the occurrence and development of exercise-induced fatigue. Method： Twenty-four male Wistar rats were randomly divided into control group （CG）, immediately after exhaustion group （EG）, and 90min after recovery group （RG）, each consists of 8 rats. The expression level of 5-HT1B and D1DR receptors in each group were observed with the immunohistochemistry technique. Result： Compared with CG, the positive cell area/scanning area value （R value） and the average optical density value （AOD value） of 5-HT1B receptor in SNr and GPi of the EG and RG rats both decreased significantly （P〈0.05）, while the R value and AOD value of D1DR both increased significantly（P〈0.05）. The R value and AOD value of 5-HT1B receptor in VL of EG and RG rats increased significantly（P〈0.05, P〈0.01）. In SMA, the R value and AOD value of 5-HT1B receptor of EG and RG rats increased significantly （P〈0.05）, while, the R value and AOD value of D1DR receptor decreased significantly （P〈0.05）. Conclusion： Changes of 5-HT1B receptor and D1DR receptor expression in the nuclei of ‘SNr/GPi-VL-SMA’ pathway caused the changes of the neurotransmitters release, thus affecting the excitability of the electrical activity of neurons, this might be one of the important ways for ‘SNr/GPi-VL-SMA’ pathway modulating the exercise-induced fatigue and exercise performance.

关 键 词：大鼠 力竭运动 “黑质/苍白球-丘脑-皮质”通路 5-羟色胺1B受体 多巴胺 型受体 

分 类 号：R338[医药卫生—人体生理学]