上转换发光免疫层析法快速检测牛奶中雌二醇  

An Up-converting Phosphor Technology Based Immunostrip Assay for Rapid Detection of Estradiol in Milk

作　　者：姜会聪 任舒悦 王瑜[2] 李巧凤[1] 白家磊[2] 彭媛[2] 宁保安[2] 孙思明[2] 高志贤[1,2] 

机构地区：[1]郑州大学公共卫生学院,河南郑州450001 [2]军事医学科学院卫生学环境医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050

出　　处：《食品研究与开发》2017年第3期119-123,共5页Food Research and Development

基　　金：国家自然科学基金(21477162);天津市科技支撑计划项目(14ZCZDSF00021);天津市应用基础与前沿技术研究计划(15JCYBJC51200)

摘　　要：建立定量快速检测雌二醇(E2)的上转换免疫层析技术。以上转换发光材料(UCP)为新型标记物,采用戊二醛法偶联单克隆抗体(m Ab),制备UPT-m Ab复合物,并以其作为探针,基于小分子竞争结合,建立免疫层析试纸条方法,并对试纸条性能进行评价。方法特异性强,稳定性好,灵敏度高,最低检出限低至2.75 ng/m L,线性范围为5 ng/m L^2 000 ng/m L,回收率88.88%~103.5%,变异系数CV<10%。成功构建了上转换免疫层析法用于检测雌二醇,适用于现场快速检测,具有良好的经济价值和应用前景。To develop an up-converting phosphor technology based immunostrip assay for quantitative detection of estradiol. A composition contained up-converting phosphor（UCP）as a novel lable and estradiol antibody（E2-m Ab）with glutaraldehyde method. The estradiol was detected by the fluorescent immunostrip assay based on indirect competitive principle which had this composition as a probe. Detection performance including stability,specificity and sensitivity was evaluated. The method showed well stability,specificity and sensitivity with the detection limit of 2.75 ng/m L,the linear range of 5 ng/m L to 2 000 ng/m L,the recovery of 88.88 % to 103.5 %,and the coefficient of variation（CV）less than 10 %. The established assay for E2 detection can be successfully used to fast-field analysis and has good economic value and broad application prospect.

关 键 词：上转换发光材料 免疫层析 雌二醇 间接竞争 

分 类 号：O658.1[理学—分析化学] TS252.7[理学—化学]