草鱼鱼鳞胶原蛋白提取及活性肽制备研究  

Extraction of Collagen from Fish Scale of Grass Carp and Preparation of Bioactive Peptide

作　　者：贺江[1] 赵自龙[1] 彭磊[1] 杨浩[1] 黄冰[1] 

机构地区：[1]湖南文理学院生命科学学院,环洞庭湖水产健康养殖与加工湖南省重点实验室,湖南省水产高效健康生产协同创新中心,湖南常德415000

出　　处：《食品研究与开发》2017年第5期52-55,共4页Food Research and Development

基　　金：湖南省常德市联合基金项目(2015JJ5008);国家自然科学基金项目(31401583)

摘　　要：以草鱼鱼鳞为原材料,探讨其胶原蛋白酸-酶组合提取工艺,并进一步研究相应抗氧化活性肽和血管紧张素转换酶抑制(ACEI)活性肽的制备方法。结果表明,草鱼鱼鳞胶原蛋白提取的最优工艺为:乙酸浓度0.3 mol/L、乙酸提取固液比1∶15(g/m L)条件下低温下提取24 h;离心后剩余残渣再按1∶10(g/m L)的比例浸入浓度为2%的胃蛋白酶溶液中低温下提取48 h;在该条件下鱼鳞胶原蛋白提取得率可达49.58%。以草鱼胶原蛋白粗提液为原料,制备抗氧化活性肽的最优工艺为:控制胶原蛋白浓度12.5 mg/m L,按10∶1(体积比)的比例加入浓度为4%的木瓜蛋白酶,60℃下酶解75 min,所得酶解液对ABTS^+自由基的抑制率可达45.7%;制备ACEI活性肽的最优工艺为:控制胶原蛋白浓度20 mg/m L,pH值调节为4.0,每10 m L加入6 000 U的酸性蛋白酶,37℃酶解3 h,所得酶解液对血管紧张素转换酶(ACE)的抑制活性可达98.8%。本研究结果,有望为水产品加工副产物的资源化利用提供参考。Fish scale of grass carp was used as row material for collagen extraction, and then the extracts were used for antioxidative peptide and angiotensin converting enzyme inhibitory peptide preparation. The optimal process of collagen extraction was as follow： acetic acid （0.3 mol/L） was added with the ratio of 1 ： 15 （g/mL） and extracted for 24 h under low temperature, then centrifugated and the residues were further extracted for 48 hours by 2 % protease with the ratio of 1 ： 10 （g/mL）. By this process, the extraction rate of collagen was 49.58 %. The optimal process of antioxidative peptide preparation was as follow： collagen concentration was adjusted to 12.5 mg/mL, 4 % papain solution was added with the volume ratio of 10 ： 1, and then enzymatic hydrolysis for 75 minutes under 60 ℃. The inhibition ratio of the end product to ATBS＋ free radical was 45.7 %. The optimal process of angiotensin converting enzyme inhibitory peptide preparation was as follow： collagen concentration was adjusted to 20 mg/mL and pH to 4.0, 6 000 U acid proteinase was added to 10 mL collagen solution, and then enzymatic hydrolysis for 3 hours under 37 ℃. The inhibition ratio of the end product to angiotensin converting enzyme was up to 98.8 %. These results can provide some reference for resource utilization of the byproducts of aquatic product processing.

关 键 词：鱼鳞 胶原蛋白 抗氧化肽 血管紧张素转换酶抑制肽 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]